Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 85.1% residual activity at 1 mM | Malus domestica | |
Mn2+ | 87.3% residual activity at 1 mM | Malus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 130% relative activity at 1 mM | Malus domestica | |
Mg2+ | 111% relative activity at 1 mM | Malus domestica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
SDS-PAGE | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonate + H2O | Malus domestica | - |
oligogalacturonates | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
cultivar Golden Delicious | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sephacryl S-200 gel filtration, and CM-Sephadex gel filtration | Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Malus domestica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0319 | - |
crude extract, in 100 mM sodium acetate, pH 5.5, containing 0.4% polygalacturonic acid, at 20°C | Malus domestica |
0.102 | - |
after purification, in 100 mM sodium acetate, pH 5.5, containing 0.4% polygalacturonic acid, at 20°C | Malus domestica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polygalacturonate + H2O | - |
Malus domestica | oligogalacturonates | - |
? | |
sodium polypectate + H2O | - |
Malus domestica | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endopolygalacturonase | - |
Malus domestica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
2 | 75 | polygalacturonase is highly active at 20 and 37°C but is also detectable at 2, 50, and 75°C. No activity is detected following incubation in boiling water for 15 s | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 5 | - |
Malus domestica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | - |
Malus domestica |
General Information | Comment | Organism |
---|---|---|
physiological function | polygalacturonase plays a role in Penicillium solitum-mediated decay of apple fruit | Malus domestica |