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Literature summary for 3.2.1.15 extracted from
- Cloning, characterization, and functional analysis of the EPG1-2 gene: a new allele coding for an endopolygalacturonase in Kluyveromyces marxianus (2009), J. Agric. Food Chem., 57, 8921-8926.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris | Kluyveromyces marxianus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
estimated from amino acid sequence | Kluyveromyces marxianus |
| 37000 | - |
SDS-PAGE | Kluyveromyces marxianus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | Q6TA03 | - |
- |
| Kluyveromyces marxianus CECT1043 | Q6TA03 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the recombinant enzyme does not appear to be fully glycosylated by Pichia pastoris or not glycosylated in the same manner as in Kluyveromyces marxianus | Kluyveromyces marxianus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate + H2O | - |
Kluyveromyces marxianus | oligogalacturonates | - |
? |  |
| polygalacturonate + H2O | - |
Kluyveromyces marxianus CECT1043 | oligogalacturonates | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endopolygalacturonase | - |
Kluyveromyces marxianus |
| Epg1-2p | - |
Kluyveromyces marxianus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Kluyveromyces marxianus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 60 | - |
Kluyveromyces marxianus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 65 | about 10% relative activity at 20°C, about 30% relative activity at 30°C, about 40% relative activity at 40°C, about 70% relative activity at 50°C, about 60% relative activity at 60°C, about 40% relative activity at 65°C | Kluyveromyces marxianus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Kluyveromyces marxianus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5.5 | - |
Kluyveromyces marxianus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | less than 10% relative activity at pH 3.0-3.5, 40% relative activity at pH 4.0, about 70% relative activity at pH 5.5, less than 10% relative activity at pH 6.0 | Kluyveromyces marxianus |
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