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Literature summary for 3.2.1.15 extracted from

  • Aminzadeh, S.; Naderi-Manesh, H.; Khajeh, K.; Ranjbar, B.; Farrokhi, N.
    Characterization of acid-induced partially folded conformation resembling a molten globule state of polygalacturonase from a filamentous fungus Tetracoccosporium sp. (2010), Appl. Biochem. Biotechnol., 160, 1921-1932.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Tetracoccosporium sp.
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-
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Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose column chromatography and Mono-Q Sepharose column chromatography Tetracoccosporium sp.

Renatured (Commentary)

Renatured (Comment) Organism
in order to assess the reversibility of acid-induced unfolding, PG at pH 1.0 is diluted 30fold by 20 mM acetate buffer, pH 4.3. Within a short time of incubation, 0 to 25 min, a refolding yield greater than 50% is measured Tetracoccosporium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
polygalacturonate + H2O
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Tetracoccosporium sp. oligogalacturonates
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