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Literature summary for 3.2.1.15 extracted from
- Functional characterization of a gene family encoding polygalacturonases in Phytophthora parasitica (2008), Mol. Plant Microbe Interact., 21, 480-489.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | highly induced during plant infection | Phytophthora parasitica |
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | endoPG genes play important roles in the pathogenesis of Phytophthora parasitica. Each gene causes specific effects, varying from no symptoms to dwarfism, necrosis, leaf curl, silvery leaf, and cracks in leaf stalks. Appearance of these effects depends on the expression of a endoPG protein with a normal active site in the apoplast. Each gene plays a distinct role in the decomposition of plant cell wall | Phytophthora parasitica |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris. PVX as a vector to systemically express endoPG in Nicotiana benthamiana, the coding sequence downstream of the signal peptide of endoPG gene subcloned into pgR106:SP, in-frame with the signal peptide of tobacco PR1a, for secretion of the recombinant protein into the apoplast | Phytophthora parasitica |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 35000 | - |
sequence analysis | Phytophthora parasitica |
| 36000 | - |
sequence analysis | Phytophthora parasitica |
| 37000 | - |
sequence analysis | Phytophthora parasitica |
| 39000 | - |
sequence analysis | Phytophthora parasitica |
| 40000 | - |
sequence analysis | Phytophthora parasitica |
| 40000 | - |
x * 40000, SDS-PAGE | Phytophthora parasitica |
| 43000 | - |
x * 43000, SDS-PAGE | Phytophthora parasitica |
| 50000 | - |
sequence analysis | Phytophthora parasitica |
| 75000 | - |
x * 75000, SDS-PAGE | Phytophthora parasitica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phytophthora parasitica | C5GZ69 | - |
- |
| Phytophthora parasitica | C5GZ70 | - |
- |
| Phytophthora parasitica | C5GZ71 | - |
- |
| Phytophthora parasitica | C5GZ72 | - |
- |
| Phytophthora parasitica | C5GZ73 | - |
- |
| Phytophthora parasitica | C5GZ74 | - |
- |
| Phytophthora parasitica | C5GZ75 | - |
- |
| Phytophthora parasitica | C5GZ76 | - |
- |
| Phytophthora parasitica | C5GZ77 | - |
- |
| Phytophthora parasitica | Q5C997 | - |
- |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 43000, SDS-PAGE | Phytophthora parasitica |
| ? | x * 40000, SDS-PAGE | Phytophthora parasitica |
| ? | x * 75000, SDS-PAGE | Phytophthora parasitica |
| ? | x * 43000-50000, SDS-PAGE | Phytophthora parasitica |
| ? | x * 45000-55000, SDS-PAGE | Phytophthora parasitica |
| ? | x * 55000-70000, SDS-PAGE | Phytophthora parasitica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endoPG | - |
Phytophthora parasitica |
| endopolygalacturonases | - |
Phytophthora parasitica |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Phytophthora parasitica | - |
- |
4 |
| Phytophthora parasitica | - |
- |
4.1 |
| Phytophthora parasitica | - |
- |
5 |
| Phytophthora parasitica | - |
- |
5.2 |
| Phytophthora parasitica | - |
- |
5.9 |
| Phytophthora parasitica | - |
- |
6.2 |
| Phytophthora parasitica | - |
- |
8.9 |
| Phytophthora parasitica | - |
- |
9.3 |
| Phytophthora parasitica | - |
- |
9.5 |
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