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Literature summary for 3.2.1.15 extracted from

  • Huang, L.; Cao, J.; Zhang, A.; Ye, Y.; Zhang, Y.; Liu, T.
    The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development (2009), J. Exp. Bot., 60, 301-313.
    View publication on PubMedView publication on EuropePMC
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Activating Compound

Activating Compound Comment Organism Structure
additional information expression of BcMF2 reaches its maximum in the stage IV flower buds Brassica rapa subsp. chinensis

Application

Application Comment Organism
additional information BcMF2 (Brassica campestris male fertility 2) gene may encode a new polygalacturonase with an important role in pollen wall development, possibly via regulation of pectin’s dynamic metabolism Brassica rapa subsp. chinensis

Cloned(Commentary)

Cloned (Comment) Organism
BcMF2 cloned in the antisense orientation into the binary vector pBI121 and transferred into the Chinese cabbage Brassica rapa subsp. chinensis

Inhibitors

Inhibitors Comment Organism Structure
antisense RNA inhibits BcMF2 expression, which results in abnormal pollen tube growth and consequent reduction in male fertility. BcMF2 inhibition leads to pollen deformities with abnormal intine development and in early degradation of the anther tapetum Brassica rapa subsp. chinensis

Organism

Organism UniProt Comment Textmining
Brassica rapa subsp. chinensis
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Source Tissue

Source Tissue Comment Organism Textmining
additional information no BcMF2 expression in the leaves or scapes of stage II or stage I flower buds prior to meiosis Brassica rapa subsp. chinensis
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pollen transcription of BcMF2 first occurrs in the stage III flower buds Brassica rapa subsp. chinensis
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