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Literature summary for 3.2.1.15 extracted from

  • Verlent, I.; Hendrickx, M.; Verbeyst, L.; Van Loey, A.
    Effect of temperature and pressure on the combined action of purified tomato pectinmethylesterase and polygalacturonase in presence of pectin (2007), Enzyme Microb. Technol., 40, 1141-1146.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
additional information tomato polygalacturonase activity is influenced indirectly by the presence of active tomato pectinmethylesterase and vice versa during thermal and high-pressure treatment Solanum lycopersicum

Application

Application Comment Organism
food industry to preserve or improve rheological properties of tomato based products, the combination of 40°C and 400MPa represents an optimal condition to reduce tomato PG activity while maintaining sufficient pectinmethylesterase activity Solanum lycopersicum

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
3.02fold Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit red ripe tomatoes Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.59
-
crude extract Solanum lycopersicum
16.87
-
3.02fold purified enzyme Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O apple pectin Solanum lycopersicum ?
-
?
polygalacturonic acid + H2O
-
Solanum lycopersicum ?
-
?

Synonyms

Synonyms Comment Organism
PG2
-
Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
optimal temperature for activity of purified tomato PG in presence of highly esterified pectin and purified tomato pectinmethylesterase at atmospheric pressure Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4
-
optimal pH for activity of purified tomato PG in presence of highly esterified pectin and purified tomato pectinmethylesterase at atmospheric pressure Solanum lycopersicum