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Literature summary for 3.2.1.15 extracted from

  • Pedrolli, D.B.; Gomes, E.; Monti, R.; Carmona, E.C.
    Studies on productivity and characterization of polygalacturonase from Aspergillus giganteus submerged culture using citrus pectin and orange waste (2008), Appl. Biochem. Biotechnol., 144, 191-200.
    View publication on PubMed
Search for more literature for 3.2.1.15

Activating Compound

Activating Compound Comment Organism Structure
additional information greater production of PG activity in shaking than in standing culture Aspergillus giganteus

Application

Application Comment Organism
food industry the enzyme has a considerable potential for commercial application, primarily in the food and animal feedstock industries, due to features such as its optimum activity in acid medium, which remains at a high level at neutral pH, and good pH and temperature stability. The utilization of orange waste in PG production leads to an increase in yield with a reduction in process cost. Moreover, it adds value to the waste from the orange juice industry Aspergillus giganteus

Organism

Organism UniProt Comment Textmining
Aspergillus giganteus
-
-
-
Aspergillus giganteus CCT 3232
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.28
-
 with apple pomace as the carbon source Aspergillus giganteus
3.01
-
 with galacturonic acid as the carbon source Aspergillus giganteus
4.81
-
 with orange waste as the carbon source Aspergillus giganteus
7.24
-
 with polygalacturonic acid as the carbon source Aspergillus giganteus
7.35
-
 with citrus pectin as the carbon source Aspergillus giganteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
citrus pectin + H2O
-
 Aspergillus giganteus ?
-
 ?
citrus pectin + H2O
-
 Aspergillus giganteus CCT 3232 ?
-
 ?
additional information no activity with glucose, sugarcane bagasse, oat bran and wheat bran Aspergillus giganteus ?
-
 ?
additional information no activity with glucose, sugarcane bagasse, oat bran and wheat bran Aspergillus giganteus CCT 3232 ?
-
 ?
polygalacturonic acid + H2O
-
 Aspergillus giganteus ?
-
 ?
polygalacturonic acid + H2O
-
 Aspergillus giganteus CCT 3232 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 highest extracellular activity on citrus pectin as sole carbon source obtained in 4.5-day-old cultures and on orange waste in 3.5-day-old cultures. Optimal polygalacturonase activity in assays conducted at 55-60°C Aspergillus giganteus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 30
-
 Aspergillus giganteus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 after 50 min of incubation in citrus pectin, it maintains 80% of its activity, half-life at 90 min. Half-life in orange waste is only 10 min Aspergillus giganteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5
-
 highest extracellular activity on citrus pectin as sole carbon source obtained in 4.5-day-old cultures. On orange bagasse, highest extracellular activity obtained in 3.5-day-old cultures at pH 6.0. Optimal polygalacturonase activity in assays conducted at pH 5.5-6.5 Aspergillus giganteus

pH Range

pH Minimum pH Maximum Comment Organism
3 10 with pectin as the carbon source Aspergillus giganteus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 8 more than 90% of PG activity remains after 24 h in citrus pectin and orange waste, incubation in orange waste gives better results than incubation in citrus pectin at extreme pHs Aspergillus giganteus