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Literature summary for 3.2.1.15 extracted from
- Purification and biochemical characterization of polygalacturonase II produced in semi-solid medium by a strain of Fusarium moniliforme (2004), Microbiol. Res., 159, 305-314.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km-value for polygalacturonate is 0.166 mg/ml | Fusarium verticillioides |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Fusarium verticillioides | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30200 | - |
gel filtration | Fusarium verticillioides |
| 30600 | - |
1 * 30600, SDS-PAGE | Fusarium verticillioides |
| 30600 | - |
x * 30600, SDS-PAGE | Fusarium verticillioides |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Fusarium verticillioides | - |
- |
- |
| Fusarium verticillioides NCIM 1276 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 8.6% carbohydrate | Fusarium verticillioides |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Fusarium verticillioides |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Fusarium verticillioides | - |
| culture medium | semi-solid | Fusarium verticillioides | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 18.66 | - |
- |
Fusarium verticillioides |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate + H2O | - |
Fusarium verticillioides | ? | - |
? |  |
| polygalacturonate + H2O | - |
Fusarium verticillioides NCIM 1276 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 30600, SDS-PAGE | Fusarium verticillioides |
| monomer | 1 * 30600, SDS-PAGE | Fusarium verticillioides |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PG II | - |
Fusarium verticillioides |
| polygalacturonase II | - |
Fusarium verticillioides |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Fusarium verticillioides |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.7 | - |
Polygalacturonate | pH 5.0, 40°C | Fusarium verticillioides | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.3 | - |
- |
Fusarium verticillioides |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Fusarium verticillioides | - |
- |
8.6 |
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Release 2023.1 (January 2023)
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