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Literature summary for 3.2.1.15 extracted from
- Production and partial characterization of an endopolygalacturonase from Saccharomyces cerevisiae (1994), Can. J. Microbiol., 40, 974-977.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| galacturonate | - |
Saccharomyces cerevisiae |  |
| Hg2+ | HgCl2 | Saccharomyces cerevisiae | |
| phosphoric acid | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Saccharomyces cerevisiae | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
CECT1389 | - |
| Saccharomyces cerevisiae CECT1389 | - |
CECT1389 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polygalacturonate + H2O | - |
Saccharomyces cerevisiae | oligogalacturonates | - |
? | |
| polygalacturonate + H2O | - |
Saccharomyces cerevisiae CECT1389 | oligogalacturonates | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Saccharomyces cerevisiae |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 60 | 20°C: about 40% of maximal activity, 60°C: about 25% of maximal activity | Saccharomyces cerevisiae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | stable | Saccharomyces cerevisiae |
| 60 | - |
inactivation in 20 min | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.3 | 6 | about 85% of maximal activity at pH 4.3 and pH 6.0 | Saccharomyces cerevisiae |
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