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Literature summary for 3.2.1.15 extracted from

  • Kimura, H.; Uchino, F.; Mizushima, S.
    Properties of a polygalacturonase produced by Acrocylindrium (1973), J. Gen. Microbiol., 74, 127-137.
No PubMed abstract available
Search for more literature for 3.2.1.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Acrocylindrium sp.
8.4
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Acrocylindrium sp.

Organism

Organism UniProt Comment Textmining
Acrocylindrium sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Acrocylindrium sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Acrocylindrium sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Acrocylindrium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O hydrolysis is undetectable Acrocylindrium sp. D-galacturonic acid
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O hydrolyis is undetectable Acrocylindrium sp. digalacturonic acid + galacturonate
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Acrocylindrium sp. alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + D-galacturonate
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Acrocylindrium sp. alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate or tetragalacturonic acid + galacturonate ?
polygalacturonate + H2O
-
 Acrocylindrium sp. oligogalacturonates
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 polygalacturonic acid protects the enzyme from heat inactivation Acrocylindrium sp.
40
-
 stable up to Acrocylindrium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2
-
-
 Acrocylindrium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 7.5 30°C, 24 h, stable Acrocylindrium sp.
9
-
 30°C, 24 h, 50% inactivation Acrocylindrium sp.