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Literature summary for 3.2.1.15 extracted from

  • Heinrichova, K.; Rexova-Benkova, L.
    Purification and characterization of another D-galacturonase from Aspergillus niger (1977), Collect. Czech. Chem. Commun., 42, 2569-2576.
No PubMed abstract available
Search for more literature for 3.2.1.15

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0196
-
 pectate
-
 Aspergillus niger
1.85
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Aspergillus niger
3.31
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Aspergillus niger
12.5
-
 alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 Aspergillus niger

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 gel filtration Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
commercial preparation commercial preparation of Koch-Light Ltd. Colnbrook, England Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
75.4
-
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Aspergillus niger digalacturonic acid + galacturonate
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Aspergillus niger alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + D-galacturonate
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Aspergillus niger alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 ?
alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + H2O
-
 Aspergillus niger alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate + alpha-D-galacturonosyl-(1,4)-O-alpha-D-galacturonate
-
 ?
pectic acid + H2O
-
 Aspergillus niger galacturonic acid oligomers
-
 ?
polygalacturonate + H2O
-
 Aspergillus niger oligogalacturonates
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Aspergillus niger

pH Range

pH Minimum pH Maximum Comment Organism
3.5 6 pH 3.5: about 30% of maximal activity, pH 6.0: about 30% of maximal activity Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.8
-
 2 h, about 60% loss of activity Aspergillus niger
3.5
-
 2 h, about 25% loss of activity Aspergillus niger
5
-
 2 h, stable Aspergillus niger
6
-
 2 h, about 10% loss of activity Aspergillus niger
7
-
 2 h, about 40% loss of activity Aspergillus niger
8
-
 2 h, about 70% loss of activity Aspergillus niger