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Literature summary for 3.2.1.147 extracted from

  • Roman, J.; Castillo, A.; Cottet, L.; Mahn, A.
    Kinetic and structural study of broccoli myrosinase and its interaction with different glucosinolates (2018), Food Chem., 254, 87-94 .
    View publication on PubMed
Search for more literature for 3.2.1.147

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis Brassica oleracea var. italica

Inhibitors

Inhibitors Comment Organism Structure
glucoraphanin substrate inhibition, kinetics, overview Brassica oleracea var. italica
additional information broccoli myrosinase subunit has two substrate-binding sites: a catalytic site where the substrate is more related to the enzyme and the residues that hydrolyze the substrate, and a second binding site with lower affinity for the substrate that might be an inhibitory site. The molecular simulations confirm the hypothesis of substrate inhibition through a two-binding site mechanism suggested by the kinetic data Brassica oleracea var. italica
sinigrin substrate inhibition, kinetics, overview Brassica oleracea var. italica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics modeling: Michaelis-Menten model (M-M), two-binding sites model (TBSM), and Michaelis-Menten with substrate inhibition (MMSI) model. The TBSM represents more accurately the system behaviour, with the highest R2 values, equal to 97.3% for glucoraphanin and 98.4% for sinigrin Brassica oleracea var. italica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucoraphanin + H2O Brassica oleracea var. italica
-
 sulforaphane + D-glucose
-
 ?
sinigrin + H2O Brassica oleracea var. italica
-
 (1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
 ?

Organism

Organism UniProt Comment Textmining
Brassica oleracea var. italica A0A343IQS8
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from inflorescences by ammonium sulfate fractionation, dialysis, concanavalin A affinity chromatography, and gel filtration Brassica oleracea var. italica

Source Tissue

Source Tissue Comment Organism Textmining
inflorescence
-
 Brassica oleracea var. italica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucoraphanin + H2O
-
 Brassica oleracea var. italica sulforaphane + D-glucose
-
 ?
additional information substrate docking analysis. modeling of glucoraphanin and sinigrin into the regulatory and the active site, three-dimensional structure model of broccoli myrosinase, modeling and validation, overview Brassica oleracea var. italica ?
-
 ?
sinigrin + H2O
-
 Brassica oleracea var. italica (1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
 ?

Synonyms

Synonyms Comment Organism
myrosinase
-
 Brassica oleracea var. italica

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Brassica oleracea var. italica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Brassica oleracea var. italica

General Information

General Information Comment Organism
additional information broccoli myrosinase subunit has two substrate-binding sites: a catalytic site where the substrate is more related to the enzyme and the residues that hydrolyze the substrate, and a second binding site with lower affinity for the substrate that might be an inhibitory site. The molecular simulations confirm the hypothesis of substrate inhibition through a two-binding site mechanism suggested by the kinetic data Brassica oleracea var. italica