Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, combining proteomics and transcriptomics analysis, phylogenetic analysis | Phyllotreta striolata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phyllotreta striolata | A0A059UAD7 | - |
- |
Purification (Comment) | Organism |
---|---|
native enzyme by three chromatographic step involving gel filtration | Phyllotreta striolata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-butenylglucosinolate + H2O | - |
Phyllotreta striolata | ? | - |
? | |
4-methylsulfinylbutylglucosinolate + H2O | - |
Phyllotreta striolata | ? | - |
? | |
4-pentenylglucosinolate + H2O | - |
Phyllotreta striolata | ? | - |
? | |
allyl glucosinolate + H2O | - |
Phyllotreta striolata | ? | - |
? | |
additional information | highest myrosinase activity is detected with allylglucosinolate. Activity with 3-butenylglucosinolate, 4-pentenylglucosinolate, and 4-methylsulfinylbutyl glucosinolate is 50-70% of that with allylglucosinolate, overview | Phyllotreta striolata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
myrosinase | - |
Phyllotreta striolata |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycoside hydrolase family 1 and has up to 76% sequence similarity to other beta-glucosidases, phylogenetic analyses. Species-specific diversification of this gene family in insects and an independent evolution of the beetle myrosinase from other insect beta-glucosidases | Phyllotreta striolata |
physiological function | adult beetles selectively accumulate glucosinolates from their food Brassicaceae plants to up to 1.75% of their body weight and express their own myrosinase, GC-MS analyses, overview. Glucosinolates of different classes accumulate at divergent rates | Phyllotreta striolata |