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Literature summary for 3.2.1.147 extracted from
- Properties of beta-thioglucoside hydrolases (TGG1 and TGG2) from leaves of Arabidopsis thaliana (2012), Plant Sci., 191-192, 82-92.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | inhibitory at over 0.7 mM | Arabidopsis thaliana |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene tgg1, exxpression of recombinant TGG1, sequence comparisons | Arabidopsis thaliana |
| gene tgg2, recombinant expression of TGG2, sequence comparisons | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | inhibitory at over 0.7 mM; inhibitory at over 0.7 mM | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0111 | - |
sinigrin | recombinant enzyme TGG2, pH 5.5, 37°C | Arabidopsis thaliana | |
| 0.0144 | - |
sinigrin | recombinant enzyme TGG1, pH 5.5, 37°C | Arabidopsis thaliana | |
| 0.05 | - |
sinigrin | recombinant enzyme TGG2, pH 5.5, 37°C, in presence of ascorbate | Arabidopsis thaliana | |
| 0.108 | - |
sinigrin | recombinant enzyme TGG1, pH 5.5, 37°C, in presence of ascorbate | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61100 | - |
x * 75000, glycosylated enzyme TGG1, SDS-PAGE, x * 61100, deglycosylated enzyme TGG1, SDS-PAGE | Arabidopsis thaliana |
| 62700 | - |
x * 63000, glycosylated enzyme TGG2, SDS-PAGE, x * 62700, deglycosylated enzyme TGG2, SDS-PAGE | Arabidopsis thaliana |
| 63000 | - |
x * 63000, glycosylated enzyme TGG2, SDS-PAGE, x * 62700, deglycosylated enzyme TGG2, SDS-PAGE | Arabidopsis thaliana |
| 75000 | - |
x * 75000, glycosylated enzyme TGG1, SDS-PAGE, x * 61100, deglycosylated enzyme TGG1, SDS-PAGE | Arabidopsis thaliana |
| 126000 | - |
gel filtration | Arabidopsis thaliana |
| 150000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | myrosinases catalyze the hydrolysis of glucosinolates, a structurally distinct group of nitrogen- and sulfur-containing secondary metabolites | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | P37702 | gene tgg1 | - |
| Arabidopsis thaliana | Q9C5C2 | gene tgg2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | deglycosylation under nondenaturing conditions has limited effect on TGG1 activity | Arabidopsis thaliana |
| glycoprotein | deglycosylation under nondenaturing conditions has no effect on TGG2 activity | Arabidopsis thaliana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| TGG1 51.9fold from from T-DNA insertion lines of Arabidopsis using lectin affinity and anion exchange chromatography | Arabidopsis thaliana |
| TGG2 84.2fold from from T-DNA insertion lines of Arabidopsis using lectin affinity and anion exchange chromatography | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| aerial part | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 91 | - |
purified recombinant enzyme TGG2, pH 5.5, 37°C | Arabidopsis thaliana |
| 99.6 | - |
purified recombinant enzyme TGG1, pH 5.5, 37°C | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-hydroxy-3-butenylglucosinolate + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
| 2-phenylethylglucosinolate + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
| 4-methylsulfinylbutylglucosinolate + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
| 4-methylthiobutylglucosinolate + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
| benzylglucosinolate + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
| additional information | substrate specificity, overview | Arabidopsis thaliana | ? | - |
? | |
| additional information | myrosinases catalyze the hydrolysis of glucosinolates, a structurally distinct group of nitrogen- and sulfur-containing secondary metabolites | Arabidopsis thaliana | ? | - |
? | |
| sinigrin + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, glycosylated enzyme TGG2, SDS-PAGE, x * 62700, deglycosylated enzyme TGG2, SDS-PAGE | Arabidopsis thaliana |
| ? | x * 75000, glycosylated enzyme TGG1, SDS-PAGE, x * 61100, deglycosylated enzyme TGG1, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-thioglucoside glucohydrolase | - |
Arabidopsis thaliana |
| myrosinase | - |
Arabidopsis thaliana |
| TGG | - |
Arabidopsis thaliana |
| TGG1 | - |
Arabidopsis thaliana |
| TGG2 | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 58 | 70% of maximal activity between 37°C and 58°C | Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 4 | 55 | purified recombinant enzyme TGG1, retains over 70% activity at temperatures between 4°C and 55°C for 30 min | Arabidopsis thaliana |
| 4 | 55 | purified recombinant enzyme TGG2, retains over 70% activity at temperatures between 4°C and 55°C for 30 min | Arabidopsis thaliana |
| 95 | - |
purified recombinant enzyme, 10 min, pH 5.8, inactivation | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
sinigrin | recombinant enzyme TGG2, pH 5.5, 37°C | Arabidopsis thaliana | |
| 0.38 | - |
sinigrin | recombinant enzyme TGG1, pH 5.5, 37°C | Arabidopsis thaliana | |
| 6.04 | - |
sinigrin | recombinant enzyme TGG2, pH 5.5, 37°C, in presence of ascorbate | Arabidopsis thaliana | |
| 10.7 | - |
sinigrin | recombinant enzyme TGG1, pH 5.5, 37°C, in presence of ascorbate | Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Arabidopsis thaliana |
| 6 | - |
- |
Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | activity range | Arabidopsis thaliana |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 11 | the purified recombinant enzyme TGG1 retains more than 50% of their maximal activity in buffers with pH values ranging from 4.8 to 11.0 | Arabidopsis thaliana |
| 4.8 | 10.3 | the purified recombinant enzyme TGG1 retains more than 50% of their maximal activity in buffers with pH values ranging from 4.8 to 10.3 | Arabidopsis thaliana |
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