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Literature summary for 3.2.1.147 extracted from

  • Wade, K.L.; Ito, Y.; Ramarathnam, A.; Holtzclaw, W.D.; Fahey, J.W.
    Purification of active myrosinase from plants by aqueous two-phase counter-current chromatography (2015), Phytochem. Anal., 26, 47-53.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
x * 61000, SDS-PAGE Raphanus sativus

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
var. italica
-
Moringa oleifera
-
-
-
Raphanus sativus
-
-
-
Sinapis alba
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from freeze-dried 3-day-old broccoli sprouts 5.51fold for mobile enzyme fraction, and 14.6fold from counter-current chromatography Brassica oleracea
native enzyme from freeze-dried 7-day-old daikon sprouts 3.45fold for mobile enzyme fraction, and 22.9fold from counter-current chromatography Raphanus sativus
native enzyme from freeze-dried leaves 2.20fold for mobile enzyme fraction, and 61.5fold from counter-current chromatography Moringa oleifera
native enzyme from seeds 4.54fold for mobile enzyme fraction, and 41.8fold from counter-current chromatography Sinapis alba

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Moringa oleifera
-
seed
-
Sinapis alba
-
sprout
-
Raphanus sativus
-
sprout
-
Brassica oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.018
-
plant crude extract, pH and temperature not specified in the publication Moringa oleifera
0.04
-
mobile enzyme fraction, pH and temperature not specified in the publication Moringa oleifera
0.058
-
plant crude extract, pH and temperature not specified in the publication Sinapis alba
0.059
-
plant crude extract, pH and temperature not specified in the publication Brassica oleracea
0.069
-
plant crude extract, pH and temperature not specified in the publication Raphanus sativus
0.238
-
mobile enzyme fraction, pH and temperature not specified in the publication Raphanus sativus
0.263
-
mobile enzyme fraction, pH and temperature not specified in the publication Sinapis alba
0.322
-
mobile enzyme fraction, pH and temperature not specified in the publication Brassica oleracea
0.85
-
counter-current chromatography fraction, pH and temperature not specified in the publication Brassica oleracea
1.12
-
counter-current chromatography fraction, pH and temperature not specified in the publication Moringa oleifera
1.58
-
counter-current chromatography fraction, pH and temperature not specified in the publication Raphanus sativus
2.42
-
counter-current chromatography fraction, pH and temperature not specified in the publication Sinapis alba

Subunits

Subunits Comment Organism
? x * 61000, SDS-PAGE Raphanus sativus

Synonyms

Synonyms Comment Organism
myrosinase
-
Raphanus sativus
myrosinase
-
Brassica oleracea
myrosinase
-
Sinapis alba
myrosinase
-
Moringa oleifera

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Raphanus sativus
6
-
assay at Brassica oleracea
6
-
assay at Sinapis alba
6
-
assay at Moringa oleifera

General Information

General Information Comment Organism
physiological function myrosinase catalyses the formation of isothiocyanates such as sulforaphane (from broccoli) and 4-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate (from moringa), which are potent inducers of the cytoprotective phase-2 response in humans, by hydrolysis of their abundant glucosinolate (beta-thioglucoside N-hydroxysulfate) precursors Raphanus sativus
physiological function myrosinase catalyses the formation of isothiocyanates such as sulforaphane (from broccoli) and 4-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate (from moringa), which are potent inducers of the cytoprotective phase-2 response in humans, by hydrolysis of their abundant glucosinolate (beta-thioglucoside N-hydroxysulfate) precursors Brassica oleracea
physiological function myrosinase catalyses the formation of isothiocyanates such as sulforaphane (from broccoli) and 4-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate (from moringa), which are potent inducers of the cytoprotective phase-2 response in humans, by hydrolysis of their abundant glucosinolate (beta-thioglucoside N-hydroxysulfate) precursors Sinapis alba
physiological function myrosinase catalyses the formation of isothiocyanates such as sulforaphane (from broccoli) and 4-(alpha-L-rhamnopyranosyloxy)benzyl isothiocyanate (from moringa), which are potent inducers of the cytoprotective phase-2 response in humans, by hydrolysis of their abundant glucosinolate (beta-thioglucoside N-hydroxysulfate) precursors Moringa oleifera