Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris | Carica papaya |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NaCl | enzyme activity is progressively inhibited by NaCl addition to the reactions and is completely inhibited at 1 M NaCl | Carica papaya |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.82 | - |
sinigrin | pH not specified in the publication, at 37°C | Carica papaya |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
x * 65000, SDS-PAGE | Carica papaya |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Carica papaya | C9WCQ0 | cultivar Solo II | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Carica papaya |
Purification (Comment) | Organism |
---|---|
immobilized metal affinity chromatography | Carica papaya |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Carica papaya | - |
leaf | - |
Carica papaya | - |
seed | - |
Carica papaya | - |
stem | - |
Carica papaya | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-nitrophenyl beta-D-glucopyranoside + H2O | - |
Carica papaya | 2-nitrophenol + D-glucopyranose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Carica papaya | 4-nitrophenol + D-glucopyranose | - |
? | |
glucotropaeolin + H2O | - |
Carica papaya | ? | - |
? | |
additional information | recombinant TGG1 has weak ascorbic acid-independent O-beta-glucosidase activity with substrate specificity | Carica papaya | ? | - |
? | |
additional information | the enzyme is inactive towards glucovanillin and n-octyl-beta-D-glucopyranoside | Carica papaya | ? | - |
? | |
sinigrin + H2O | - |
Carica papaya | D-glucose + isothiocyanate + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 65000, SDS-PAGE | Carica papaya |
Synonyms | Comment | Organism |
---|---|---|
beta-thioglucosidase | - |
Carica papaya |
myrosinase | - |
Carica papaya |
TGG1 | - |
Carica papaya |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Carica papaya |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
65.3 | - |
sinigrin | pH not specified in the publication, at 37°C | Carica papaya |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Carica papaya |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | more than 60% of maximum activity around pH 5.5 and 9.0 | Carica papaya |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is ascorbic acid-independent | Carica papaya |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
sinigrin | pH not specified in the publication, at 37°C | Carica papaya |