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Literature summary for 3.2.1.147 extracted from

  • Nong, H.; Zhang, J.M.; Li, D.Q.; Wang, M.; Sun, X.P.; Zhu, Y.J.; Meijer, J.; Wang, Q.H.
    Characterization of a novel beta-thioglucosidase CpTGG1 in Carica papaya and its substrate-dependent and ascorbic acid-independent O-beta-glucosidase activity (2010), J. Integr. Plant Biol., 52, 879-890.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Carica papaya

Inhibitors

Inhibitors Comment Organism Structure
NaCl enzyme activity is progressively inhibited by NaCl addition to the reactions and is completely inhibited at 1 M NaCl Carica papaya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.82
-
sinigrin pH not specified in the publication, at 37°C Carica papaya

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
x * 65000, SDS-PAGE Carica papaya

Organism

Organism UniProt Comment Textmining
Carica papaya C9WCQ0 cultivar Solo II
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Carica papaya

Purification (Commentary)

Purification (Comment) Organism
immobilized metal affinity chromatography Carica papaya

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Carica papaya
-
leaf
-
Carica papaya
-
seed
-
Carica papaya
-
stem
-
Carica papaya
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-nitrophenyl beta-D-glucopyranoside + H2O
-
Carica papaya 2-nitrophenol + D-glucopyranose
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
-
Carica papaya 4-nitrophenol + D-glucopyranose
-
?
glucotropaeolin + H2O
-
Carica papaya ?
-
?
additional information recombinant TGG1 has weak ascorbic acid-independent O-beta-glucosidase activity with substrate specificity Carica papaya ?
-
?
additional information the enzyme is inactive towards glucovanillin and n-octyl-beta-D-glucopyranoside Carica papaya ?
-
?
sinigrin + H2O
-
Carica papaya D-glucose + isothiocyanate + ?
-
?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Carica papaya

Synonyms

Synonyms Comment Organism
beta-thioglucosidase
-
Carica papaya
myrosinase
-
Carica papaya
TGG1
-
Carica papaya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Carica papaya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
65.3
-
sinigrin pH not specified in the publication, at 37°C Carica papaya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 8.5
-
Carica papaya

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9 more than 60% of maximum activity around pH 5.5 and 9.0 Carica papaya

Cofactor

Cofactor Comment Organism Structure
additional information the enzyme is ascorbic acid-independent Carica papaya

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
23
-
sinigrin pH not specified in the publication, at 37°C Carica papaya