Literature summary for 3.2.1.147 extracted from

Andersson, D.; Chakrabarty, R.; Bejai, S.; Zhang, J.; Rask, L.; Meijer, J.
Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties (2009), Phytochemistry, 70, 1345-1354.

Search for more literature for 3.2.1.147

Activating Compound

Activating Compound	Comment	Organism	Structure
ascorbic acid	the myrosinase isozymes show different activationinhibition responses towards ascorbic acid with maximal activity around 0.7-1 mM	Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression of His-tagged root isozymes TGG4 and TGG5 and His-tagged leaf isozyme TGG1 in Pichia pastoris	Arabidopsis thaliana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.045	-	sinigrin	pH 5.5, isozyme TGG1	Arabidopsis thaliana
0.245	-	sinigrin	pH 5.5, isozyme TGG4	Arabidopsis thaliana
0.547	-	sinigrin	pH 5.5, isozyme TGG5	Arabidopsis thaliana
28	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.5, isozyme TGG4	Arabidopsis thaliana
34	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.5, isozyme TGG1	Arabidopsis thaliana
80	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.5, isozyme TGG5	Arabidopsis thaliana

Metals/Ions

Metals/Ions	Comment	Organism	Structure
additional information	the isozymes are active in a wide range of salt concentrations but sensitive to high salt concentrations	Arabidopsis thaliana

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	-	six myrosinase genes, TGG1-TGG6	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged root isozymes TGG4 and TGG5 and His-tagged leaf isozyme TGG1 from Pichia pastoris by nickel affinity chromatography to homogeneity	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
leaf	isozymne TGG1	Arabidopsis thaliana	-
root	isozymnes TGG4 and TGG5	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl beta-D-glucopyranoside + H2O	-	Arabidopsis thaliana	4-nitrophenol + D-glucopyranose	-	?
additional information	differences in basal activity of myrosinase isozymes, no activity with desulfosinigrin	Arabidopsis thaliana	?	-	?
sinigrin + H2O	i.e. 2-propenyl glucosinolate	Arabidopsis thaliana	allyl isothiocyanate + D-glucose + ?	-	?

Synonyms

Synonyms	Comment	Organism
myrosinase	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
40	-	isozyme TGG1	Arabidopsis thaliana

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	60	isozyme TGG1, 65% of maximal activity at 20°C, progressive increase that peaks at 40°C, high activity at 50°C, inactivation above 60°C	Arabidopsis thaliana
20	80	20% of maximal activity at 20°C, progressive increase that peaks at 70°C, 30% of maximal activity at 80°C	Arabidopsis thaliana
20	90	20% of maximal activity at 20°C, progressive increase that peaks at 60°C, 5% of maximal activity at 90°C	Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	the isozymes are rather thermostable	Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.2	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.5, isozyme TGG1	Arabidopsis thaliana
2.3	-	sinigrin	pH 5.5, isozyme TGG1	Arabidopsis thaliana
7.3	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.5, isozyme TGG4	Arabidopsis thaliana
12	-	sinigrin	pH 5.5, isozyme TGG4	Arabidopsis thaliana
17	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.5, isozyme TGG5	Arabidopsis thaliana
46	-	sinigrin	pH 5.5, isozyme TGG5	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	isozyme TGG5	Arabidopsis thaliana
5.5	10.5	isozymes TGG1 and TGG4	Arabidopsis thaliana

pH Range

pH Minimum	pH Maximum	Comment	Organism
2.5	12	isozymes TGG4 and TGG5 are active at pH 2.5, and TGG4 shows a broad optimum at pH 5.5-10.5, while TGG5 peaks at pH 5.5 and than loses activity at pH 6.5 and 8.5, both show 50% of maximal activity at pH 11.5	Arabidopsis thaliana
4.5	12	isozyme TGG1, inactive below pH 4.5, rather unaffected by differences in pH above 5.5	Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.035	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.5, isozyme TGG1	Arabidopsis thaliana
0.21	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.5, isozyme TGG5	Arabidopsis thaliana
0.26	-	4-nitrophenyl beta-D-glucopyranoside	pH 5.5, isozyme TGG4	Arabidopsis thaliana
48	-	sinigrin	pH 5.5, isozyme TGG4	Arabidopsis thaliana
53	-	sinigrin	pH 5.5, isozyme TGG1	Arabidopsis thaliana
85	-	sinigrin	pH 5.5, isozyme TGG5	Arabidopsis thaliana

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Release 2023.1 (January 2023)