Literature summary for 3.2.1.147 extracted from

Borgen, B.H.; Thangstad, O.P.; Ahuja, I.; Rossiter, J.T.; Bones, A.M.
Removing the mustard oil bomb from seeds: transgenic ablation of myrosin cells in oilseed rape (Brassica napus) produces MINELESS seeds (2010), J. Exp. Bot., 61, 1683-1697.

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Cloned(Commentary)

Cloned (Comment)	Organism
recombinant gene Myr1.Bn1, expressionin transgenic Brassica napus plants	Brassica napus

Protein Variants

Protein Variants	Comment	Organism
additional information	genetic modification of Brassica napus plants to remove myrosinase-storing idioblasts to eliminate release of cell toxic reaction products and metabolites. Construction of transgenic plants ectopically expressing barnase, a ribonuclease, using a seed myrosin cell-specific Myr1.Bn1 promoter, which is lethal for the embryo. Co-expressing barnase under the control of the Myr1.Bn1 promoter with the barnase inhibitor, barstar, under the control of the cauliflower mosaic virus 35S promoter enables a selective and controlled death of myrosin cells without affecting plant viability. Transgenic plants with myrosin defence cells show negligible production of glucosinolate hydrolysis products and altered epithiospecifier protein profile and glucosinolate levels, overview. Glucosinolate profiles of wild-type and mutant plants, overview	Brassica napus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Brassica napus	MYR is a beta-thioglucosidase that hydrolyses glucosinolates to a variety of products such as isothiocyanates, thiocyanates, nitriles, epithionitriles, and oxazolidine-thiones depending on the nature of the glucosinolates. Glucosinolates are themselves biologically inactive, but glucosinolates hydrolytic products, such as thiocyanates, isothiocyanates, nitriles, and oxazolidine-2-thione, produced by MYR during processing of oilseed rape meal are biologically active. Glucosinolate profiles of wild-type and mutant plants, overview	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Brassica napus	Q9STD7	recombinant gene Myr1.Bn1; cv. Westar, gene Myr1	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
cotyledon	-	Brassica napus	-
idioblast	myrosinase-storing cells, i.e. myrosin cells	Brassica napus	-
seed	-	Brassica napus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	MYR is a beta-thioglucosidase that hydrolyses glucosinolates to a variety of products such as isothiocyanates, thiocyanates, nitriles, epithionitriles, and oxazolidine-thiones depending on the nature of the glucosinolates. Glucosinolates are themselves biologically inactive, but glucosinolates hydrolytic products, such as thiocyanates, isothiocyanates, nitriles, and oxazolidine-2-thione, produced by MYR during processing of oilseed rape meal are biologically active. Glucosinolate profiles of wild-type and mutant plants, overview	Brassica napus	?	-	?
sinigrin + H2O	i.e. 2-propenyl glucosinolate	Brassica napus	allyl isothiocyanate + D-glucose + ?	-	?

Synonyms

Synonyms	Comment	Organism
MYR	-	Brassica napus
myrosinase	-	Brassica napus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5.5	-	assay at	Brassica napus

General Information

General Information	Comment	Organism
physiological function	myrosinase-catalysed release of toxic and bioactive compounds such as isothiocyanates, upon activation or tissue damage, play a role in the plant defense system	Brassica napus

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Release 2023.1 (January 2023)