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Literature summary for 3.2.1.147 extracted from

  • Kissen, R.; Bones, A.M.
    Nitrile-specifier proteins involved in glucosinolate hydrolysis in Arabidopsis thaliana (2009), J. Biol. Chem., 284, 12057-12070.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.147

Cloned(Commentary)

Cloned (Comment) Organism
overexpression of nitrile-specifier protein 2, NSP2, in transgenic Arabidopsis thaliana plants leading to increased nitrile production Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ shifts the enzyme to nitril fomration with increasing concentration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabidopsis thaliana the degradation of glucosinolates is catalyzed by thioglucosidases called myrosinases and leads by default to the formation of isothiocyanates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxy-3-butenylglucosinolate + H2O
-
 Arabidopsis thaliana 5-vinyl-2-oxazolidine thione + D-glucose
-
 ?
2-phenylethylglucosinolate + H2O
-
 Arabidopsis thaliana 2-phenylethyl-isothiocyanate + D-glucose
-
 ?
2-propenylglucosinolate + H2O epithiospecifier protein and nitrile-specifier protein can switch myrosinase-catalyzed degradation of 2-propenylglucosinolate from isothiocyanate to nitrile, only epithiospecifier protein generates the corresponding epithionitrile Arabidopsis thaliana 2-propenyl-isothiocyanate + D-glucose
-
 ?
3-benzyloxypropylglucosinolate + H2O
-
 Arabidopsis thaliana 3-benzyloxypropyl-isothiocyanate + D-glucose
-
 ?
3-butenylglucosinolate + H2O
-
 Arabidopsis thaliana 3-butenyl-isothiocyanate + D-glucose
-
 ?
3-methylthiopropylglucosinolate + H2O
-
 Arabidopsis thaliana 3-methylthiopropyl-isothiocyanate + D-glucose
-
 ?
4-benzyloxybutylglucosinolate + H2O
-
 Arabidopsis thaliana 4-benzyloxybutyl-isothiocyanate + D-glucose
-
 ?
4-methylsulfinylbutylglucosinolate + H2O
-
 Arabidopsis thaliana 4-methylsulfinylbutyl-isothiocyanate + D-glucose
-
 ?
4-methylthiobutylglucosinolate + H2O
-
 Arabidopsis thaliana 4-methylthiobutyl-isothiocyanate + D-glucose
-
 ?
4-pentenylglucosinolate + H2O
-
 Arabidopsis thaliana 4-pentenyl-isothiocyanate + D-glucose
-
 ?
5-methylthiopentylglucosinolate + H2O
-
 Arabidopsis thaliana 5-methylthiopentyl-isothiocyanate + D-glucose
-
 ?
6-methylthiohexylglucosinolate + H2O
-
 Arabidopsis thaliana 6-methylthiohexyl-isothiocyanate + D-glucose
-
 ?
7-methylthioheptylglucosinolate + H2O
-
 Arabidopsis thaliana 7-methylthioheptyl-isothiocyanate + D-glucose
-
 ?
8-methylthiooctylglucosinolate + H2O
-
 Arabidopsis thaliana 8-methylthiooctyl-isothiocyanate + D-glucose
-
 ?
benzylglucosinolate + H2O nitrile-specifier proteins, especially nitrile-specifier protein 2, NSP2, in conjunction with myrosinase enable the enzyme to generate nitriles, overview Arabidopsis thaliana benzylisothiocyanate + D-glucose + ?
-
 ?
indolyl-3-methylglucosinolate + H2O
-
 Arabidopsis thaliana indolyl-3-methyl-isothiocyanate + D-glucose
-
 ?
additional information the degradation of glucosinolates is catalyzed by thioglucosidases called myrosinases and leads by default to the formation of isothiocyanates Arabidopsis thaliana ?
-
 ?
additional information myrosinase acts on glucosinolates to form an unstable aglycone intermediate that can rearrange spontaneously to form an isothiocyanate. Interaction of a protein called epithiospecifier protein with myrosinase diverts the reaction toward the production of epithionitriles or nitriles depending on the glucosinolate structure, while nitrile-specifier proteins, especially nitrile-specifier protein 2, NSP2, enable to generate nitriles in conjunction with myrosinase, tissue distributions of the specifier proteins, overview Arabidopsis thaliana ?
-
 ?

Synonyms

Synonyms Comment Organism
myrosinase
-
 Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction the degradation of glucosinolates is catalyzed by thioglucosidases called myrosinases and leads by default to the formation of isothiocyanates Arabidopsis thaliana