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Literature summary for 3.2.1.147 extracted from
- Characterization of a Brassica napus myrosinase expressed and secreted by Pichia pastoris (2002), Protein Expr. Purif., 24, 221-226.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | 1 mM, 100fold activation | Brassica napus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression and secretion by Pichia pastoris | Brassica napus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
sinigrin | 30°C, pH 6.8 | Brassica napus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Brassica napus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
x * 64000, SDS-PAGE | Brassica napus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Brassica napus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | recombinant enzyme is secreted by Pichia pastoris | Brassica napus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 175 | - |
- |
Brassica napus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for several months | Brassica napus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sinigrin + H2O | - |
Brassica napus | glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, SDS-PAGE | Brassica napus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MYR1 myrosinase | - |
Brassica napus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 187 | - |
sinigrin | 30°C, pH 6.8 | Brassica napus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | - |
Brassica napus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | 8 | pH 5.2: about 45% of maximal activity, pH 8.0: about 60% of maximal activity | Brassica napus |
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