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Literature summary for 3.2.1.147 extracted from
- Isolation and biochemical characterization of a basic myrosinase from ripe Crambe abyssinica seeds, highly specific for epi-progoitrin (2003), J. Agric. Food Chem., 51, 2737-2744.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | activates | Crambe hispanica subsp. abyssinica |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.14 | - |
progoitrin | 37°C, without ascorbic acid | Crambe hispanica subsp. abyssinica | |
| 0.17 | - |
sinigrin | 37°C, without ascorbic acid | Crambe hispanica subsp. abyssinica | |
| 0.24 | - |
epi-progoitrin | 37°C, without ascorbic acid | Crambe hispanica subsp. abyssinica | |
| 0.43 | - |
epi-progoitrin | 37°C, with 20 mM ascorbic acid | Crambe hispanica subsp. abyssinica | |
| 1.1 | - |
progoitrin | 37°C, with 20 mM ascorbic acid | Crambe hispanica subsp. abyssinica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
6 * 75000, SDS-PAGE | Crambe hispanica subsp. abyssinica |
| 470000 | - |
gel filtration | Crambe hispanica subsp. abyssinica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crambe hispanica subsp. abyssinica | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | carbohydrate chains distributed over the surface of the enzyme | Crambe hispanica subsp. abyssinica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Crambe hispanica subsp. abyssinica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 44 | 66 | - |
Crambe hispanica subsp. abyssinica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| epi-progoitrin + H2O | - |
Crambe hispanica subsp. abyssinica | ? | - |
? | |
| progoitrin + H2O | - |
Crambe hispanica subsp. abyssinica | ? | - |
? | |
| sinigrin + H2O | - |
Crambe hispanica subsp. abyssinica | glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 75000, SDS-PAGE | Crambe hispanica subsp. abyssinica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MYRc | - |
Crambe hispanica subsp. abyssinica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
hydrolysis of epiprogoitrin | Crambe hispanica subsp. abyssinica |
| 8.5 | - |
hydrolysis of progoitrin | Crambe hispanica subsp. abyssinica |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | pH 5.0: about 70% of maximal activity, pH 10: about 40% of maximal activity, hydrolysis of progoitrin | Crambe hispanica subsp. abyssinica |
| 5 | 8.5 | pH 5.0: about 40% of maximal activity, pH 8.5: about 45% of maximal activity, hydrolysis of epiprogoitrin | Crambe hispanica subsp. abyssinica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 11 | 37°C, 30 min, stable | Crambe hispanica subsp. abyssinica |
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