General Stability | Organism |
---|---|
some loss of activity upon freezing | Brevicoryne brassicae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | 0.3 mM, strong activation | Brevicoryne brassicae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
sinigrin | 37°C, pH 4.5 | Brevicoryne brassicae | |
0.52 | - |
glucotropaeolin | 37°C, pH 4.5 | Brevicoryne brassicae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
126000 | - |
sucrose density gradient sedimentation | Brevicoryne brassicae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brevicoryne brassicae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Brevicoryne brassicae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28 | - |
- |
Brevicoryne brassicae |
Storage Stability | Organism |
---|---|
4°C, stable for more than 6 months | Brevicoryne brassicae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glucotropaeolin + H2O | - |
Brevicoryne brassicae | ? | - |
? | |
p-nitrophenyl beta-D-glucopyranoside + H2O | - |
Brevicoryne brassicae | p-nitrophenol + D-glucose | - |
? | |
sinigrin + H2O | - |
Brevicoryne brassicae | D-glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 57000-58000, SDS-PAGE | Brevicoryne brassicae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
hydrolysis of sinigrin | Brevicoryne brassicae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3 | 6 | sinigrin | 37°C, pH 4.5 | Brevicoryne brassicae | |
22.8 | - |
glucotropaeolin | 37°C, pH 4.5 | Brevicoryne brassicae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Brevicoryne brassicae | isoelectric focusing, pH-range 3-9 | - |
4.9 |