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Literature summary for 3.2.1.147 extracted from

  • El-Sayed, S.T.; Jwanny, E.W.
    Glycosidases of turnip leaf tissues (1994), Appl. Biochem. Biotechnol., 49, 11-22.
No PubMed abstract available
Search for more literature for 3.2.1.147

Activating Compound

Activating Compound Comment Organism Structure
EDTA 0.04 M, 70% activation Brassica rapa

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Brassica rapa
Ca2+
-
 Brassica rapa
Cu2+
-
 Brassica rapa
Pb2+
-
 Brassica rapa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
 sinigrin
-
 Brassica rapa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 gel filtration Brassica rapa

Organism

Organism UniProt Comment Textmining
Brassica rapa
-
 subsp. rapa
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brassica rapa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O
-
 Brassica rapa D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Brassica rapa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 60 min, 24% loss of activity Brassica rapa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Brassica rapa

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.5: about 40% of maximal activity, pH 7.0: about 70% of maximal activity Brassica rapa