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Literature summary for 3.2.1.147 extracted from
- The crystal structures of Sinapis alba myrosinase and a covalent glycosyl-enzyme intermediate provide insight into the substrate recognition and active-site machinery of an S-glycosidase (1997), Structure, 5, 663-675.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| native myrosinase and stable glycosyl-enzyme intermediate | Sinapis alba |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | dimeric enzyme is stabilized by a Zn2+-ion bound on a twofold axis. The Zn2+ has a tetrahedral coordination, with angles between 97° and 118°, though four residues: His56 and Asp70, and their symmetry-related equivalents | Sinapis alba |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sinapis alba | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 13000 Da carbohydrate per dimer, the carbohydrate is required to maintain molecular stability and solubility in the dehydrated environment of the seed | Sinapis alba |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Sinapis alba | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sinigrin + H2O | - |
Sinapis alba | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
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