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Literature summary for 3.2.1.147 extracted from
- Development and characteristics of myrosinase in Brassica napus during early seedling growth (1991), Physiol. Plant., 82, 163-170.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | activates | Brassica napus |  |
| ascorbic acid | maximal activation for myrosinase I, 50fold at 0.3 mM. Maximal activation for myrosinase II, 106fold between 0.3 and 0.5 mM | Brassica napus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
3 * 62000, myrosinase II, SDS-PAGE | Brassica napus |
| 75000 | - |
2 * 75000, myrosinase I, SDS-PAGE | Brassica napus |
| 156000 | - |
myrosinase I, gel filtration | Brassica napus |
| 188000 | - |
myrosinase II, gel filtration | Brassica napus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | - |
L. cv. Bienvenu | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| myrosinase I and II | Brassica napus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Brassica napus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 114 | - |
myrosinase I | Brassica napus |
| 283 | - |
myrosinase II | Brassica napus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzylglucosinolate + H2O | - |
Brassica napus | additional information | - |
? | |
| glucobrassicin + H2O | - |
Brassica napus | ? | - |
? | |
| neo-glucobrassicin + H2O | - |
Brassica napus | ? | - |
? | |
| phenylethylglucosinolate + H2O | - |
Brassica napus | ? | - |
? | |
| progoitrin + H2O | - |
Brassica napus | additional information | - |
? | |
| sinigrin + H2O | - |
Brassica napus | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 75000, myrosinase I, SDS-PAGE | Brassica napus |
| trimer | 3 * 62000, myrosinase II, SDS-PAGE | Brassica napus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6 | myrosinase I and II | Brassica napus |
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