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Literature summary for 3.2.1.147 extracted from

  • Tani, N.; Ohtsuru, M.; Hata, T.
    Purification and general characteristics of bacterial myrosinase produced by Enterobacter cloacae (1974), Agric. Biol. Chem., 38, 1623-1630.
No PubMed abstract available
Search for more literature for 3.2.1.147

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 1 mM, activates Enterobacter cloacae

Inhibitors

Inhibitors Comment Organism Structure
1-O-methyl-alpha-D-glucopyranose
-
 Enterobacter cloacae
amygdalin
-
 Enterobacter cloacae
arbutin
-
 Enterobacter cloacae
ascorbic acid 0.1 mM L-ascorbic acid, 88% inhibition Enterobacter cloacae
Ca2+ 1 mM, CaCl2, 14% inhibition Enterobacter cloacae
Co2+ 1 mM, CoCl2, 27% inhibition Enterobacter cloacae
Cu+ 1 mM, CuCl, 56% inhibition Enterobacter cloacae
Cu2+ 1 mM, CuCl2, 78% inhibition Enterobacter cloacae
delta-gluconolactone
-
 Enterobacter cloacae
EDTA
-
 Enterobacter cloacae
Fe2+ 1 mM, FeCl2, 68% inhibition Enterobacter cloacae
Fe3+ 1 mM, FeCl3, 18% inhibition Enterobacter cloacae
fructose
-
 Enterobacter cloacae
galactose
-
 Enterobacter cloacae
glucose
-
 Enterobacter cloacae
HgCl2 1 mM, HgCl2, 87% inhibition Enterobacter cloacae
maltose
-
 Enterobacter cloacae
mannose
-
 Enterobacter cloacae
methyl-beta-D-glucopyranoside
-
 Enterobacter cloacae
Mg2+ 1 mM, MgCl2, 22% inhibition Enterobacter cloacae
Ni2+ 1 mM, NiCl2, 30% inhibition Enterobacter cloacae
p-nitrophenyl-beta-D-glucopyranoside
-
 Enterobacter cloacae
phenyl-beta-D-glucopyranoside
-
 Enterobacter cloacae
Salicin
-
 Enterobacter cloacae
sorbitol
-
 Enterobacter cloacae
Sr2+ 1 mM, SrCl2, 21% inhibition Enterobacter cloacae
sucrose
-
 Enterobacter cloacae
Zn2+ 1 mM, ZnCl2, 45% inhibition Enterobacter cloacae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 sinigrin
-
 Enterobacter cloacae
0.71
-
 p-nitrophenyl beta-D-glucopyranoside
-
 Enterobacter cloacae

Metals/Ions

Metals/Ions Comment Organism Structure
Li+ 1 mM LiCl2, activates Enterobacter cloacae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 gel filtration Enterobacter cloacae

Organism

Organism UniProt Comment Textmining
Enterobacter cloacae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11.5
-
-
 Enterobacter cloacae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl beta-D-glucopyranoside + H2O
-
 Enterobacter cloacae ?
-
 ?
sinigrin + H2O
-
 Enterobacter cloacae D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 pH 6.8, 30 min, stable Enterobacter cloacae
40
-
 pH 6.8, 30 min, about 20% loss of activity Enterobacter cloacae
50
-
 pH 6.8, 30 min, about 50% loss of activity Enterobacter cloacae
60
-
 pH 6.8, 30 min, about 90% loss of activity Enterobacter cloacae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
-
 Enterobacter cloacae

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.5 pH 5.5: about 40% of maximal activity, pH 7.5: about 60% of maximal activity Enterobacter cloacae

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 below 40°C, 24 h, stable Enterobacter cloacae