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Literature summary for 3.2.1.147 extracted from

  • Ohtsuru, M.; Kawatani, H.
    Studies on the myrosinase from Wasabia japonica: purification and some properties of Wasabi myrosinase (1979), Agric. Biol. Chem., 43, 2249-2255.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
ascorbic acid active only in presence of L-ascorbic acid, maximal activation at 2 mM Eutrema japonicum
ascorbyl palmitate lower activation than with ascorbic acid Eutrema japonicum
ascorbyl stearate lower activation than with ascorbic acid Eutrema japonicum
D-araboascorbate lower activation than with ascorbic acid Eutrema japonicum

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Eutrema japonicum
2-methoxy-5-nitrotropone
-
Eutrema japonicum
5,5'-dithiobis(2-nitrobenzoic acid)
-
Eutrema japonicum
Cu2+ 1 mM CuCl2, complete inhibition Eutrema japonicum
EDTA
-
Eutrema japonicum
Fe3+ 1 mM FeCl3, 16% inhibition Eutrema japonicum
HgCl2 1 mM HgCl2, 93% inhibition Eutrema japonicum
p-mercuribenzoate
-
Eutrema japonicum
Trinitrobenzenesulfonic acid
-
Eutrema japonicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.47
-
sinigrin
-
Eutrema japonicum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 1 mM CaCl2, activates Eutrema japonicum
Co2+ 1 mM CoCl2, activates Eutrema japonicum
K+ 1 mM KCl2, activates Eutrema japonicum
Mg2+ 1 mM MgCl2, slight activation Eutrema japonicum
Mn2+ 1 mM MnCl2, activates Eutrema japonicum
Na+ 1 mM NaCl2, slight activation Eutrema japonicum
Ni2+ 1 mM NiCl2, activates Eutrema japonicum
Sn2+ 1 mM SnCl2, activates Eutrema japonicum
Sr2+ 1 mM SrCl2, activates Eutrema japonicum
Zn2+ 1 mM ZnCl2, activates Eutrema japonicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
580000
-
gel filtration Eutrema japonicum

Organism

Organism UniProt Comment Textmining
Eutrema japonicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Eutrema japonicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Eutrema japonicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O
-
Eutrema japonicum D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
?

Subunits

Subunits Comment Organism
dodecamer 12 * 45000-47000, SDS-PAGE Eutrema japonicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Eutrema japonicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 50 about 40% of maximal activity at 30°C and at 50°C Eutrema japonicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
pH 7.0, 20 min, stable Eutrema japonicum
40
-
pH 7.0, 20 min, about 25% loss of activity Eutrema japonicum
50
-
pH 7.0, 20 min, about 60% loss of activity Eutrema japonicum
70
-
pH 7.0, 20 min, about 80% loss of activity Eutrema japonicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Eutrema japonicum

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: about 45% of maximal activity, pH 9.0: about 40% of maximal activity Eutrema japonicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4
-
5°C, 41 h, about 60% loss of activity Eutrema japonicum
6
-
5°C, 41 h, about 10% loss of activity Eutrema japonicum
7
-
5°C, 41 h, stable Eutrema japonicum
10
-
5°C, 41 h, about 25% loss of activity Eutrema japonicum
11
-
5°C, 41 h, about 30% loss of activity Eutrema japonicum