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Literature summary for 3.2.1.147 extracted from

  • Petroski, R.J.; Tookey, H.L.
    Interactions of thioglucoside glucohydrolase and epithiospecifier protein of cruciferous plants to form 1-cyanoepithioalkanes (1982), Phytochemistry, 21, 1903-1905.
No PubMed abstract available
Search for more literature for 3.2.1.147

Activating Compound

Activating Compound Comment Organism Structure
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica oleracea
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica rapa
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Sinapis alba
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Crambe hispanica subsp. abyssinica
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Armoracia rusticana
epithiospecifier protein non-specific requirement for epithiospecifier protein Crambe hispanica subsp. abyssinica

Organism

Organism UniProt Comment Textmining
Armoracia rusticana
-
 i.e. horseradish
-
Brassica oleracea
-
 var. gemmifera i.e. Brussel sprouts
-
Brassica oleracea
-
 var. capitata L. f. alba DC i.e. white cabbage
-
Brassica rapa
-
 ssp. rapifera, i.e. turnip
-
Crambe hispanica subsp. abyssinica
-
-
-
Sinapis alba
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Brassica oleracea
-
leaf
-
 Brassica rapa
-
root
-
 Sinapis alba
-
root
-
 Armoracia rusticana
-
seed
-
 Brassica rapa
-
seed
-
 Sinapis alba
-
seed
-
 Crambe hispanica subsp. abyssinica
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
allylglucosinolate + H2O
-
 Brassica oleracea additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
allylglucosinolate + H2O
-
 Brassica rapa additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
allylglucosinolate + H2O
-
 Sinapis alba additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
allylglucosinolate + H2O
-
 Crambe hispanica subsp. abyssinica additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
allylglucosinolate + H2O
-
 Armoracia rusticana additional information conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein ?
sinigrin + H2O
-
 Brassica oleracea D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
sinigrin + H2O
-
 Brassica rapa D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
sinigrin + H2O
-
 Sinapis alba D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
sinigrin + H2O
-
 Crambe hispanica subsp. abyssinica D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
sinigrin + H2O
-
 Armoracia rusticana D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?