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Literature summary for 3.2.1.146 extracted from

  • Tuekam, B.A.; Park, Y.I.; Unkefer, C.J.; Gander, J.E.
    Relationship of exo-beta-D-galactofuranosidase kinetic parameters to the number of phosphodiesters in Penicillium fellutanum peptidophosphogalactomannan: enzyme purification and kinetics of glycopeptide and galactofuran chain hydrolysis (2001), Appl. Environ. Microbiol., 67, 4648-4656.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.8
-
 peptidophosphogalactomannan pP2GM 40°C, pH 4 Penicillium fellutanum
2.6
-
 1-O-methyl-beta-D-galactofuranoside 40°C, pH 4 Penicillium fellutanum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, SDS-PAGE Penicillium fellutanum
150000
-
 nondenaturing PAGE Penicillium fellutanum

Organism

Organism UniProt Comment Textmining
Penicillium fellutanum
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme contains approximately 60 mannosyl residues per mol of enzyme, based on a MW of 70000 Da Penicillium fellutanum

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium fellutanum

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Penicillium fellutanum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25
-
-
 Penicillium fellutanum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-O-(p-nitrophenyl)-beta-D-galactofuranoside + H2O
-
 Penicillium fellutanum p-nitrophenol + beta-D-galactofuranose
-
 ?
1-O-methyl-beta-D-galactofuranoside + H2O
-
 Penicillium fellutanum methanol + beta-D-galactofuranose
-
 ?
additional information no hydrolysis of: 1-O-(p-nitrophenyl)-beta-D-galactopyranoside, 1-O-(p-nitrophenyl)-alpha-D-galactopyranoside,1-O-(p-nitrophenyl)-beta-N-acetyl-D-glucopyranosylamine, p-nitrophenyl-phosphocholine, p-nitrophenyl-phosphate, or bis-(p-nitrophenyl)-phosphate Penicillium fellutanum ?
-
 ?
peptidophosphogalactomannan pP2GM + H2O
-
 Penicillium fellutanum beta-D-galactofuranose
-
 ?
tri-beta-D-(1,5)-galactofuranooligosaccharide + tetra-beta-D-(1,5)-galactofuranooligosaccharide + H2O a mixture of tri-beta-D-(1,5)-galactofuranooligosaccharide and tetra-beta-D-(1,5)-galactofuranooligosaccharide Penicillium fellutanum beta-D-galactofuranose
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, SDS-PAGE Penicillium fellutanum

Synonyms

Synonyms Comment Organism
exo-beta-D-galactofuranosidase
-
 Penicillium fellutanum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Penicillium fellutanum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 60 40°C: maximal activity, 60°C: about 60% of maximal activity Penicillium fellutanum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
 peptidophosphogalactomannan pP2GM 40°C, pH 4 Penicillium fellutanum
29
-
 1-O-methyl-beta-D-galactofuranoside 40°C, pH 4 Penicillium fellutanum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 4.5
-
 Penicillium fellutanum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 5 24°C, 24 h, optimal stability Penicillium fellutanum

pI Value

Organism Comment pI Value Maximum pI Value
Penicillium fellutanum isoelectric focusing, pH-range 4.0-6.5
-
 4.35