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Literature summary for 3.2.1.146 extracted from
- An extracellular beta-galactofuranosidase from Aspergillus niger and its use as a tool for glycoconjugate analysis (2001), Biochim. Biophys. Acta, 1525, 19-28.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-galactono-1,4-lactone | - |
Aspergillus fumigatus |  |
| D-galactono-1,4-lactone | competitive inhibition, 50% inhibition in the presence of 1 mM ZnCl2, CaCl2, NiCl2 or AgNO3 | Aspergillus niger | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.87 | - |
p-nitrophenyl beta-D-galactofuranoside | - |
Aspergillus niger | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
non-denaturing PAGE | Aspergillus niger |
| 90000 | - |
SDS-PAGE | Aspergillus niger |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus fumigatus | - |
- |
- |
| Aspergillus nidulans | - |
- |
- |
| Aspergillus niger | - |
enzyme is induced in the presence of galactofuranoside containing glycoconjugates | - |
| Aspergillus parasiticus | - |
- |
- |
| Aspergillus tamarii | - |
- |
- |
| Aspergillus versicolor | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Aspergillus niger |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| dialysed culture filtrate, Sepharose-Q, NeobarAQ-1, Neobar AQ-2, Superose-12, GNA-agarose | Aspergillus niger |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26.3 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl beta-D-galactofuranoside + H2O | - |
Aspergillus nidulans | p-nitrophenol + D-galactose | - |
? | |
| p-nitrophenyl beta-D-galactofuranoside + H2O | - |
Aspergillus niger | p-nitrophenol + D-galactose | - |
? | |
| p-nitrophenyl beta-D-galactofuranoside + H2O | - |
Aspergillus parasiticus | p-nitrophenol + D-galactose | - |
? | |
| p-nitrophenyl beta-D-galactofuranoside + H2O | - |
Aspergillus fumigatus | p-nitrophenol + D-galactose | - |
? | |
| p-nitrophenyl beta-D-galactofuranoside + H2O | - |
Aspergillus versicolor | p-nitrophenol + D-galactose | - |
? | |
| p-nitrophenyl beta-D-galactofuranoside + H2O | - |
Aspergillus tamarii | p-nitrophenol + D-galactose | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 4 | very little activity above pH 6.0 | Aspergillus niger |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | - |
Aspergillus niger |
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