Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium longum subsp. Longum | A0A401ETL2 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) | Bifidobacterium longum subsp. Longum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-1,6-Gal2-galactitol + H2O | the enzyme specifically releases beta-1,6-Gal2 but not galactose from beta-1,6-Gal2-Gat | Bifidobacterium longum subsp. Longum | beta-1,6-Gal2 + galactitol | - |
? | |
beta-1,6-Gal3 + H2O | - |
Bifidobacterium longum subsp. Longum | beta-1,6-Gal2 + D-galactose | - |
? | |
beta-1,6-galactooligosaccharides + H2O | the enzyme releases beta-1,6-Gal2 from beta-1,6-galactooligosaccharides (beta-1,6-Gal>9) in a time-dependent manner with low rates of beta-1,6-Gal3 and beta-1,6-Gal4 | Bifidobacterium longum subsp. Longum | beta-1,6-Gal2 + ? | - |
? | |
larch type II arabinogalactan + H2O | degradation profiles of type II AG | Bifidobacterium longum subsp. Longum | ? | - |
? | |
larch type II arabinogalactan + H2O | release of galactobiose, beta-1,6-Gal2, degradation profiles of type II AG | Bifidobacterium longum subsp. Longum | beta-1,6-Gal2 + ? | - |
? | |
additional information | the recombinant exo-beta-1,6-galactobiohydrolase specifically releases beta-1,6-galactobiose (beta-1,6-Gal2) from the nonreducing terminal of beta-1,6-galactooligosaccharides. beta-1,6-Gal2 is additively released from larch type II arabinogalactan (AG) by the combined use of type II AG degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145). Degradative mechanism, overview | Bifidobacterium longum subsp. Longum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 116000, recombinant His-tagged enzyme, SDS-PAGE, x * 113302, His-tagged enzyme, sequence calculation | Bifidobacterium longum subsp. Longum |
Synonyms | Comment | Organism |
---|---|---|
Bl1,3Gal | - |
Bifidobacterium longum subsp. Longum |
Bl1,6Gal | - |
Bifidobacterium longum subsp. Longum |
exo-beta-1,3-galactanase | - |
Bifidobacterium longum subsp. Longum |
exo-beta-1,6-galactobiohydrolase | - |
Bifidobacterium longum subsp. Longum |
GH30_5 exo-beta-1,6-galactobiohydrolase | - |
Bifidobacterium longum subsp. Longum |
GH43_24 exo-beta-1,3-galactanase | - |
Bifidobacterium longum subsp. Longum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
assay at | Bifidobacterium longum subsp. Longum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
assay at | Bifidobacterium longum subsp. Longum |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family 30, GH30, subfamily 22. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) forms a gene cluster with GH30_5 and GH43_22 enzyme candidates, which are conserved in all Bifidobacterium longum strains | Bifidobacterium longum subsp. Longum |
evolution | the enzyme belongs to the glycosyl hydrolase family 43, GH43, subfamily 24. GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal) forms a gene cluster with GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal) and GH43_22 alpha-L-arabinofuranosidase (BlArafA) enzyme candidates, which are conserved in all Bifidobacterium longum strains | Bifidobacterium longum subsp. Longum |
metabolism | GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) | Bifidobacterium longum subsp. Longum |
metabolism | GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55), which are degradative enzymes for type II arabinogalactan (AG) side chains in cooperation with GH43_24 exo-beta-1,3-galactanase (Bl1,3Gal, EC 3.2.1.145) | Bifidobacterium longum subsp. Longum |
physiological function | type II arabinogalactan (AG) degradative enzymes, including Bl1,3Gal, Bl1,6Gal, and BlArafA. GH30_5 exo-beta-1,6-galactobiohydrolase (Bl1,6Gal, EC 3.2.1.145) and GH43_22 alpha-L-arabinofuranosidase (BlArafA, EC 3.2.1.55) are important for the acquisition of type II AG in Bifidobacterium longum | Bifidobacterium longum subsp. Longum |