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Literature summary for 3.2.1.145 extracted from

  • Okawa, M.; Fukamachi, K.; Tanaka, H.; Sakamoto, T.
    Identification of an exo-beta-1,3-D-galactanase from Fusarium oxysporum and the synergistic effect with related enzymes on degradation of type II arabinogalactan (2013), Appl. Microbiol. Biotechnol., 97, 9685-9694.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene Fo1,3 gal, recombinant expression in Pichia pastoris strain KM71H, subcloning in Escherichia coli strain DH5alpha. The expression vector pPICZ?A contains the native Saccharomyces cerevisiae alpha-factor secretion signal, which allows Pichia pastoris to secrete heterologous proteins into the growth medium Fusarium oxysporum

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum B6F260 gene Fo1,3 gal
-
Fusarium oxysporum 12S B6F260 gene Fo1,3 gal
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble extracellular enzyme 5.7fold from Pichia pastoris strain KM71H by anion exchange and hydrophobic interaction chromatography Fusarium oxysporum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
18.9
-
purified recombinant enzyme, pH 5.0, 30°C, beta-1,3-galactan as substrate Fusarium oxysporum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,3-D-galactobiose + H2O
-
Fusarium oxysporum D-galactose
-
?
beta-1,3-D-galactobiose + H2O
-
Fusarium oxysporum 12S D-galactose
-
?
beta-1,3-galactan + H2O preferred substrate Fusarium oxysporum oligo-beta-(1->3)-D-galactose + D-galactose
-
?
beta-1,3-galactan + H2O preferred substrate Fusarium oxysporum 12S oligo-beta-(1->3)-D-galactose + D-galactose
-
?
gum arabic + H2O
-
Fusarium oxysporum ?
-
?
gum arabic + H2O
-
Fusarium oxysporum 12S ?
-
?
larch wood arabinogalactan + H2O the enzyme releases galactose, beta-1,6-D-galactobiose, beta-1,6-D-galactotriose, and beta-1,6-D-galactotriose substituted with a single arabinofuranose residue accompanied with unidentified oligosaccharides, indicating that the enzyme can by-pass the branching points of beta-1,3-galactan backbones Fusarium oxysporum ?
-
?
larch wood arabinogalactan + H2O the enzyme releases galactose, beta-1,6-D-galactobiose, beta-1,6-D-galactotriose, and beta-1,6-D-galactotriose substituted with a single arabinofuranose residue accompanied with unidentified oligosaccharides, indicating that the enzyme can by-pass the branching points of beta-1,3-galactan backbones Fusarium oxysporum 12S ?
-
?
additional information no activity of the recombinant enzyme with 4-nitrophenyl beta-D-galactopyranoside, beta-1,4-D-galactobiose, or beta-1,6-D-galactobiose, and no activity with birchwood xylan, lupin galactan, carob galactomannan, sugar beet L-arabinan, and wheat arabinoxylan Fusarium oxysporum ?
-
?
additional information no activity of the recombinant enzyme with 4-nitrophenyl beta-D-galactopyranoside, beta-1,4-D-galactobiose, or beta-1,6-D-galactobiose, and no activity with birchwood xylan, lupin galactan, carob galactomannan, sugar beet L-arabinan, and wheat arabinoxylan Fusarium oxysporum 12S ?
-
?
sugar beet debranched L-arabinan + H2O
-
Fusarium oxysporum ?
-
?
type II arabinogalactan + H2O
-
Fusarium oxysporum ?
-
?

Subunits

Subunits Comment Organism
More the enzyme contains a GH43_6 domain and a CBM35_galactosidase-like domain Fusarium oxysporum

Synonyms

Synonyms Comment Organism
exo-beta-1,3-D-galactanase
-
Fusarium oxysporum
Fo/1,3Gal
-
Fusarium oxysporum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Fusarium oxysporum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Fusarium oxysporum

General Information

General Information Comment Organism
metabolism the enzyme shows a synergistic effect with related enzymes on degradation of type II arabinogalactan Fusarium oxysporum