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Literature summary for 3.2.1.145 extracted from

  • Ichinose, H.; Kuno, A.; Kotake, T.; Yoshida, M.; Sakka, K.; Hirabayashi, J.; Tsumuraya, Y.; Kaneko, S.
    Characterization of an exo-beta -1,3-galactanase from Clostridium thermocellum (2006), Appl. Environ. Microbiol., 72, 3515-3523.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.145

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Acetivibrio thermocellus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 SDS-PAGE Acetivibrio thermocellus

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DD67 precursor
-

Purification (Commentary)

Purification (Comment) Organism
Ni-nitrilotriacetic acid agarose colum Acetivibrio thermocellus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
21.3
-
 beta-1,3-galactan as a substrate Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alpha-L-arabinofuranosidase-treated root arabinogalactan proteins from radish + H2O relative activity: 9.3 Acetivibrio thermocellus ?
-
 ?
beta-(1-3)-D-galactotriose + H2O relative activity: 119%. Enzyme hydrolyzes beta-1,3-linked galacto-oligosaccharides but does not hydrolyze beta-1,4-linked and beta-1,6-linked galacto-oligosaccharide. Ct1,3Gal43A also can not hydrolyze the beta-1,3-galactosyl linkage of beta-1,3-galactosyl galactosaminide, beta-1,3-galactosyl glucosaminide, or beta-1,3-galactosyl L-arabinofuranoside Acetivibrio thermocellus beta-(1-3)-D-galactobiose + D-galactose
-
 ?
beta-(1-3)-galactobiose + H2O relative activity: 31% Acetivibrio thermocellus D-galactose
-
 ?
beta-1,3-beta-1,6-galactan + H2O relative activity: 3.1% Acetivibrio thermocellus ?
-
 ?
beta-1,3-galactan + H2O relative activity: 100%. Enzyme catalyzes hydrolysis only of beta-1,3-linked galactosyl oligosaccharides and polysaccharides. Enzyme does not hydrolyze beta-1,4-linked galactose-containing polysaccharides, such as pectic galactan and alpha-L-arabinofuranosidase-treated pectic galactan from lupine, and also does not hydrolyze laminarin, CM-curdlan, or beta-1,3-xylan, suggesting that the enzyme acts only on a beta-1,3-linked galactan chain Acetivibrio thermocellus oligo-beta-(1->3)-D-galactose + D-galactose
-
 ?

Synonyms

Synonyms Comment Organism
Ct1,3Gal43A
-
 Acetivibrio thermocellus
exo-beta-1,3-D-galactanase
-
 Acetivibrio thermocellus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Acetivibrio thermocellus
50
-
-
 Acetivibrio thermocellus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Acetivibrio thermocellus

pH Range

pH Minimum pH Maximum Comment Organism
3 10
-
 Acetivibrio thermocellus