Cloned (Comment) | Organism |
---|---|
Escherichia coli BL21(DE3)pLysS, LB medium, vector pRSET-B | Metallosphaera hakonensis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | bifunctional fusion enzyme (MhMTSH) of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH) | Metallosphaera hakonensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
SDS-PAGE, in accordance with the calculated value from the amino acid sequence, fusion enzyme including the hexahistidine domain from the vector | Metallosphaera hakonensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera hakonensis | - |
grown on JCM165 medium at 70°C | - |
Metallosphaera hakonensis JMC8857 | - |
grown on JCM165 medium at 70°C | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
5 mM maltooligosaccharides, 100mM sodium phosphate buffer, pH 5, 70°C, 30 min, with substrate soluble starch 24 h | Metallosphaera hakonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | hydrolysis to disaccharides after 24 h | Metallosphaera hakonensis | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | hydrolysis to disaccharides after 24 h | Metallosphaera hakonensis JCM 8857 | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | hydrolysis to disaccharides after 24 h | Metallosphaera hakonensis JMC8857 | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
maltotriosyltrehalose + H2O | sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose | Metallosphaera hakonensis | maltotriose + alpha,alpha'-trehalose | - |
? | |
maltotriosyltrehalose + H2O | sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose | Metallosphaera hakonensis JCM 8857 | maltotriose + alpha,alpha'-trehalose | - |
? | |
maltotriosyltrehalose + H2O | sequential hydrolysis by the bifunctional fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), synthase converts alpha-1,4-glycosidic linkage into alpha-1,1 linkage to produce maltotriosyltrehalose, which is the substrate for the MTH to produce trehalose | Metallosphaera hakonensis JMC8857 | maltotriose + alpha,alpha'-trehalose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 150000, fusion enzyme of maltooligosyltrehalose synthase (MhMTS) and malooligosyltrehalose trehalohydrolase (MhMTH), SDS-PAGE | Metallosphaera hakonensis |
Synonyms | Comment | Organism |
---|---|---|
maltooligosyltrehalose trehalohydrolase | - |
Metallosphaera hakonensis |
MhMTH | - |
Metallosphaera hakonensis |
MTH | - |
Metallosphaera hakonensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Metallosphaera hakonensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | fusion enzyme with 5 mM maltopentaose, 30 min: 100% activity at 70, about 90% at 60, about 80% at 50 and 80, below 40% at 40 and 90°C | Metallosphaera hakonensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 90 | 80% activity retained after 48 h pre-incubation at 70°C, activities after pre-incubation at set temperatures for 30 min: 90-100% at 40-70°C, less than 80% at 80°C, quickly abolished beyond 80°C | Metallosphaera hakonensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Metallosphaera hakonensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | pH 4: about 70% activity, pH 5-6: about 100% activity, pH 7: about 40% activity, pH 8-9: between 20-30%, pH 4-5: 0.1 M McIlvaine buffer, pH 5-8: 0.1 M sodium phosphate buffer, pH 8-9: 0.1 M sodium bicarbonate buffer, 70°C, 30 min | Metallosphaera hakonensis |