Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant enzymes in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
A259S | site-directed mutagenesis, the mutant shows increased selectivity ratios, i.e. ratios of the catalytic efficiencies for glucose formation to those for trehalose formation in the hydrolysis of maltooligosaccharides and maltooligosyltrehaloses, respectively | Saccharolobus solfataricus |
D255A | site-directed mutagenesis, the mutant shows highly reduced catalytic activity compared to the wild-type MTHase | Saccharolobus solfataricus |
D380A | site-directed mutagenesis, the mutant shows highly reduced catalytic activity compared to the wild-type MTHase | Saccharolobus solfataricus |
E286A | site-directed mutagenesis, the mutant shows highly reduced catalytic activity compared to the wild-type MTHase | Saccharolobus solfataricus |
F355Y | site-directed mutagenesis, the mutant shows increased selectivity ratios, i.e. ratios of the catalytic efficiencies for glucose formation to those for trehalose formation in the hydrolysis of maltooligosaccharides and maltooligosyltrehaloses, respectively | Saccharolobus solfataricus |
R356K | site-directed mutagenesis, the mutant shows increased selectivity ratios, i.e. ratios of the catalytic efficiencies for glucose formation to those for trehalose formation in the hydrolysis of maltooligosaccharides and maltooligosyltrehaloses, respectively | Saccharolobus solfataricus |
W218A | site-directed mutagenesis, the mutant shows decreased selectivity ratios, i.e. ratios of the catalytic efficiencies for glucose formation to those for trehalose formation in the hydrolysis of maltooligosaccharides and maltooligosyltrehaloses, respectively | Saccharolobus solfataricus |
W218F | site-directed mutagenesis, the mutant shows decreased selectivity ratios, i.e. ratios of the catalytic efficiencies for glucose formation to those for trehalose formation in the hydrolysis of maltooligosaccharides and maltooligosyltrehaloses, respectively | Saccharolobus solfataricus |
Y328F | site-directed mutagenesis, the mutant shows increased selectivity ratios, i.e. ratios of the catalytic efficiencies for glucose formation to those for trehalose formation in the hydrolysis of maltooligosaccharides and maltooligosyltrehaloses, respectively | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes, overview | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharolobus solfataricus | MTHase catalyzes the release of trehalose by cleaving the alpha-1,4-glucosidic linkage next to the alpha-1,1-linked terminal disaccharide of maltooligosyltrehalose, D255, E286, and D380 are essential catalytic residues, while residues A259, Y328, F355, and R356 are related to enzyme selectivity, mutational analysis, overview | ? | - |
? | |
additional information | Saccharolobus solfataricus P2 | MTHase catalyzes the release of trehalose by cleaving the alpha-1,4-glucosidic linkage next to the alpha-1,1-linked terminal disaccharide of maltooligosyltrehalose, D255, E286, and D380 are essential catalytic residues, while residues A259, Y328, F355, and R356 are related to enzyme selectivity, mutational analysis, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | P95867 | strain KM1 and ATCC 35092 | - |
Saccharolobus solfataricus P2 | P95867 | strain KM1 and ATCC 35092 | - |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli by anion exchange chromatography and gel filtration | Saccharolobus solfataricus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.1 | - |
mutant D380 | Saccharolobus solfataricus |
0.74 | - |
mutant E286A | Saccharolobus solfataricus |
1.23 | - |
mutant D255A | Saccharolobus solfataricus |
5.31 | - |
mutant W218A | Saccharolobus solfataricus |
52 | - |
mutant W218F | Saccharolobus solfataricus |
472 | - |
mutant R356K | Saccharolobus solfataricus |
492 | - |
mutant Y328F | Saccharolobus solfataricus |
597 | - |
mutant F355Y | Saccharolobus solfataricus |
787 | - |
mutant A259S | Saccharolobus solfataricus |
814 | - |
wild-type enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | MTHase catalyzes the release of trehalose by cleaving the alpha-1,4-glucosidic linkage next to the alpha-1,1-linked terminal disaccharide of maltooligosyltrehalose, D255, E286, and D380 are essential catalytic residues, while residues A259, Y328, F355, and R356 are related to enzyme selectivity, mutational analysis, overview | Saccharolobus solfataricus | ? | - |
? | |
additional information | hydrolysis of G3T-G5T and G5-G7. Production of trehalose from starch together with isoamylase and maltooligosyltrehalose synthase, overview | Saccharolobus solfataricus | ? | - |
? | |
additional information | MTHase catalyzes the release of trehalose by cleaving the alpha-1,4-glucosidic linkage next to the alpha-1,1-linked terminal disaccharide of maltooligosyltrehalose, D255, E286, and D380 are essential catalytic residues, while residues A259, Y328, F355, and R356 are related to enzyme selectivity, mutational analysis, overview | Saccharolobus solfataricus P2 | ? | - |
? | |
additional information | hydrolysis of G3T-G5T and G5-G7. Production of trehalose from starch together with isoamylase and maltooligosyltrehalose synthase, overview | Saccharolobus solfataricus P2 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
maltooligosyltrehalose trehalohydrolase | - |
Saccharolobus solfataricus |
MTHase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Saccharolobus solfataricus |