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Literature summary for 3.2.1.140 extracted from

  • Hattie, M.; Debowski, A.W.; Stubbs, K.A.
    Development of tools to study lacto-N-biosidase: an important enzyme involved in the breakdown of human milk oligosaccharides (2012), ChemBioChem, 13, 1128-1131.
    View publication on PubMed
Search for more literature for 3.2.1.140

Cloned(Commentary)

Cloned (Comment) Organism
C-terminally His6-tagged enzyme Bifidobacterium bifidum

Inhibitors

Inhibitors Comment Organism Structure
N-acetylglucosamine-thiazoline
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 Bifidobacterium bifidum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bifidobacterium bifidum human milk oligosaccharides are substrates and sole carbon source for Bifidobacterium bifidum colonizing the human intestinal tract. More than 130 types of human milk oligosaccharides have been isolated with the most abundant being lacto-N-tetraose, lacto-N-fucopentaose I, lacto-N-difucohexaose, and 2'-fucosyllactose ?
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 ?

Organism

Organism UniProt Comment Textmining
Bifidobacterium bifidum
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-fucosyllactose + H2O Fuc-alpha-(1->2)-Gal-beta-(1->4)-Glc Bifidobacterium bifidum lactose + fucose
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 ?
lacto-N-difucohexaose + H2O Fuc-alpha-(1->2)Gal-beta-(1->3)[Fuc-alpha-(1->4)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc Bifidobacterium bifidum lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)[Fuc-alpha-(1->4)]GlcNAc
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 ?
lacto-N-fucopentaose I + H2O Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc Bifidobacterium bifidum lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc
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 ?
lacto-N-tetraose + H2O Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc Bifidobacterium bifidum lactose + lacto-N-biose
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 ?
additional information human milk oligosaccharides are substrates and sole carbon source for Bifidobacterium bifidum colonizing the human intestinal tract. More than 130 types of human milk oligosaccharides have been isolated with the most abundant being lacto-N-tetraose, lacto-N-fucopentaose I, lacto-N-difucohexaose, and 2'-fucosyllactose Bifidobacterium bifidum ?
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 ?

Synonyms

Synonyms Comment Organism
lacto-N-biosidase
-
 Bifidobacterium bifidum

General Information

General Information Comment Organism
metabolism the enzyme is critical in the lacto-N-biose pathway Bifidobacterium bifidum
additional information three realistic mechanistic alternatives exist for lacto-N-biosidases: substrate-assisted catalytic mechanism, or a mechanism involving the formation and breakdown of a covalent aglycosyl enzyme intermediate, or an inverting mechanism, overview. A key difference between these mechanistic alternatives is the involvement of the 2-acetamido group of the substrate. The lacto-N-biosidase-type enzyme uses a mechanism involving substrate-assisted catalysis from the 2-acetamido group of the substrate. Decrease in second-order rate constant, the carbonyl oxygen acts as a nucleophile, attacking the anomeric centre Bifidobacterium bifidum