Cloned (Comment) | Organism |
---|---|
C-terminally His6-tagged enzyme | Bifidobacterium bifidum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-acetylglucosamine-thiazoline | - |
Bifidobacterium bifidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bifidobacterium bifidum | human milk oligosaccharides are substrates and sole carbon source for Bifidobacterium bifidum colonizing the human intestinal tract. More than 130 types of human milk oligosaccharides have been isolated with the most abundant being lacto-N-tetraose, lacto-N-fucopentaose I, lacto-N-difucohexaose, and 2'-fucosyllactose | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-fucosyllactose + H2O | Fuc-alpha-(1->2)-Gal-beta-(1->4)-Glc | Bifidobacterium bifidum | lactose + fucose | - |
? | |
lacto-N-difucohexaose + H2O | Fuc-alpha-(1->2)Gal-beta-(1->3)[Fuc-alpha-(1->4)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | Bifidobacterium bifidum | lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)[Fuc-alpha-(1->4)]GlcNAc | - |
? | |
lacto-N-fucopentaose I + H2O | Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | Bifidobacterium bifidum | lactose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc | - |
? | |
lacto-N-tetraose + H2O | Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)-Glc | Bifidobacterium bifidum | lactose + lacto-N-biose | - |
? | |
additional information | human milk oligosaccharides are substrates and sole carbon source for Bifidobacterium bifidum colonizing the human intestinal tract. More than 130 types of human milk oligosaccharides have been isolated with the most abundant being lacto-N-tetraose, lacto-N-fucopentaose I, lacto-N-difucohexaose, and 2'-fucosyllactose | Bifidobacterium bifidum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
lacto-N-biosidase | - |
Bifidobacterium bifidum |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is critical in the lacto-N-biose pathway | Bifidobacterium bifidum |
additional information | three realistic mechanistic alternatives exist for lacto-N-biosidases: substrate-assisted catalytic mechanism, or a mechanism involving the formation and breakdown of a covalent aglycosyl enzyme intermediate, or an inverting mechanism, overview. A key difference between these mechanistic alternatives is the involvement of the 2-acetamido group of the substrate. The lacto-N-biosidase-type enzyme uses a mechanism involving substrate-assisted catalysis from the 2-acetamido group of the substrate. Decrease in second-order rate constant, the carbonyl oxygen acts as a nucleophile, attacking the anomeric centre | Bifidobacterium bifidum |