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Literature summary for 3.2.1.139 extracted from
- Isolation and characterization of a novel GH67 alpha-glucuronidase from a mixed culture (2012), J. Ind. Microbiol. Biotechnol., 39, 1245-1251.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene deg75-AG, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysE | uncultured bacterium |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 14% inhibition at 1 mM | uncultured bacterium |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | 17% activation at 1 mM | uncultured bacterium | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| uncultured bacterium | - |
gene deg75-AG | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysE by immobilized metal affinity chromatography and gell filtration | uncultured bacterium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 11.7 | - |
purified recombinant enzyme, pH 8.0, 45°C | uncultured bacterium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| birchwood xylan + H2O | - |
uncultured bacterium | ? | release of reduced sugar | ? | |
| additional information | the enzyme functions synergistically with xylanase in the hydrolysis of xylan substrate. The main products of the hydrolysis reaction are xylotriose, xylotetraose, and xylopentaose | uncultured bacterium | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DEG75-AG | - |
uncultured bacterium |
| GH67 alpha-glucuronidase | - |
uncultured bacterium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
uncultured bacterium |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 60 | activity range, profile overview | uncultured bacterium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | - |
uncultured bacterium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 9.5 | activity range, profile overview | uncultured bacterium |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | the enzyme protein sequence contains two highly conserved amino acids, D43 and E462, that are implicated as critical catalytic residues | uncultured bacterium |
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Release 2023.1 (January 2023)
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