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Literature summary for 3.2.1.139 extracted from

  • Fujimoto, Z.; Ichinose, H.; Biely, P.; Kaneko, S.
    Crystallization and preliminary crystallographic analysis of the glycoside hydrolase family 115 alpha-glucuronidase from Streptomyces pristinaespiralis (2011), Acta Crystallogr. Sect. F, 67, 68-71.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.139

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Tuner (DE3) cells Streptomyces pristinaespiralis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 20% (w/v) PEG 2000 MME and 0.1 M Tris pH 7.0 Streptomyces pristinaespiralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
111000
-
 x * 111000, SDS-PAGE Streptomyces pristinaespiralis
114000
-
 x * 114000, calculated from amino acid sequence Streptomyces pristinaespiralis

Organism

Organism UniProt Comment Textmining
Streptomyces pristinaespiralis B5H8Y8
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA agarose column chromatography, DEAE-Sepharose column chromatography, and Superose 12 gel filtration Streptomyces pristinaespiralis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
aldopentauronic acid + H2O i.e. 2''-O-alpha-(4-O-methyl-alpha-D-glucuronosyl)-xylotetraose Streptomyces pristinaespiralis 4-O-methyl-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xylbeta(1-4)Xyl
-
 ?
beechwood xylan + H2O
-
 Streptomyces pristinaespiralis 4-O-methyl-D-glucuronic acid + D-glucuronic acid + ?
-
 ?
birchwood xylan + H2O
-
 Streptomyces pristinaespiralis 4-O-methyl-D-glucuronic acid + D-glucuronic acid
-
 ?

Subunits

Subunits Comment Organism
? x * 111000, SDS-PAGE Streptomyces pristinaespiralis
? x * 114000, calculated from amino acid sequence Streptomyces pristinaespiralis

Synonyms

Synonyms Comment Organism
GlcA115A
-
 Streptomyces pristinaespiralis