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Literature summary for 3.2.1.139 extracted from

  • Iihashi, N.; Nagayama, J.; Habu, N.; Konno, N.; Isogai, A.
    Enzymatic degradation of amylouronate (alpha-(1->4)-linked glucuronan) by alpha-glucuronidase from Paenibacillus sp. TH501b (2009), Carbohydr. Polym., 77, 59-64.
No PubMed abstract available
Search for more literature for 3.2.1.139

Application

Application Comment Organism
synthesis hydrolysis of amylouronate to glucuronate by AUH-I Paenibacillus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
 1 *80000 + 1 * 46000, SDS-PAGE Paenibacillus sp.
115000
-
 native AUH-I, gel filtration Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
-
-
Paenibacillus sp. TH501b
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Toyopearl SuperQ column chromatography, Toyopearl phenyl column chromatography, Resource Q column chromatography, and TSKgel G3000SWXL gel filtration Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.07
-
 crude extract Paenibacillus sp.
2.8
-
 after 41.5fold purification Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylouronate + H2O AUH-I is highly specific to amylouronate, amylouronate is alpha-(1,4)-linked polyglucuronic acid sodium salt or alpha-(1,4)-linked glucuronan, AUH-I hydrolyses amylouronate exolytically Paenibacillus sp. D-glucuronate + ?
-
 ?
amylouronate + H2O AUH-I is highly specific to amylouronate, amylouronate is alpha-(1,4)-linked polyglucuronic acid sodium salt or alpha-(1,4)-linked glucuronan, AUH-I hydrolyses amylouronate exolytically Paenibacillus sp. TH501b D-glucuronate + ?
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 *80000 + 1 * 46000, SDS-PAGE Paenibacillus sp.

Synonyms

Synonyms Comment Organism
alpha-glucosiduronate glucuronohydrolase
-
 Paenibacillus sp.
amylouronate hydrolase-I
-
 Paenibacillus sp.
AUH-I
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Paenibacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 40
-
 Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 40 AUH-I is stable at 10-40°C, but starts to lose the activity above 40°C Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 7
-
 Paenibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8
-
 Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 almost no enzyme activity appears at pHs lower than 4.0 and higher than 9.0 Paenibacillus sp.