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Literature summary for 3.2.1.139 extracted from

  • Nong, G.; Rice, J.D.; Chow, V.; Preston, J.F.
    Aldouronate utilization in Paenibacillus sp. strain JDR-2: Physiological and enzymatic evidence for coupling of extracellular depolymerization and intracellular metabolism (2009), Appl. Environ. Microbiol., 75, 4410-4418.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.139

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap column chromatography Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-O-methyl-alpha-D-glucopyranuronosyl-(1-2)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O
-
 Paenibacillus sp. 4-O-methyl-alpha-D-glucuronic acid + beta-D-xylopyranose
-
 ?
4-O-methyl-alpha-D-glucopyranuronosyl-(1-2)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose + H2O
-
 Paenibacillus sp. 4-O-methyl-alpha-D-glucuronic acid + beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranosyl-(1-4)-beta-D-xylopyranose
-
 ?

Synonyms

Synonyms Comment Organism
AguA
-
 Paenibacillus sp.
GH67 alpha-glucuronidase
-
 Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
 at pH 6.0, using methylglucuronoxylose as substrate Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 AguA shows 54% of the maximal activity at 50°C Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 at 37°C, using methylglucuronoxylose as substrate Paenibacillus sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5
-
 AguA exhibits 85% of its maximal activity at pH 5.0 Paenibacillus sp.