Cloned (Comment) | Organism |
---|---|
Pichia stipitis CBS 6054 grown in YNB medium, cells washed and suspended in YNB medium with a xylooligosaccharide misture and methyl-beta-xylopyranoside, 30°C, 24 h, shaker | Scheffersomyces stipitis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | - |
SDS-PAGE with 2 sets of protein markers: Fermentas no. SM0431, SERVA no. 39216 | Scheffersomyces stipitis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scheffersomyces stipitis | A3LY17 | gene ABN67901, EMBL; CBS 6054 | - |
Purification (Comment) | Organism |
---|---|
supernatant concentrated on Amicon 10 kDa cut-off membranes, anion-exchange chromatography on HiTrap DEAE-FF column with NaCl gradient in 50 mM sodium-phosphate buffer, pH 7.0, fractions between 0.2 and 0.25 M NaCl pooled, concentrated, desalted, equilibrated in 50 mM acetate buffer, pH 4.0, with 2 M (NH4)2SO4, subjected to hydrophobic interaction chromatography on Butyl-FF column, eluted with (NH4)2SO4 gradient, active fractions between 1.1 and 0.61 M (NH4)2SO4 pooled, desalted, concentrated, subjected to 2 additional anion-exchange chromatography steps on Tricorn MonoQ 5/50GL column, first equilibrated with 50 mM sodium acetate buffer, pH 4.0, eluted with NaCl gradient, then equilibrated with 50 mM sodium phosphate buffer, pH 7.0 and NaCl gradient, concentration with Microcon 10 kDa cut-off membrane filter | Scheffersomyces stipitis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-O-Me-GlcAalpha(1-2)Xylbeta(1-4)Xylbeta(1-4)Xyl + H2O | from beechwood, de-estrification of the methyl ester of MeGlcA with glucuronoyl esterase | Scheffersomyces stipitis | 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl | de-estrification of the methyl ester of MeGlcA with glucuronoyl esterase | ? | |
4-O-Me-GlcAalpha(1-2)Xylbeta(1-4)Xylbeta(1-4)[4-O-Me-GlcAalpha(1-2)]Xylbeta(1-4)Xyl + H2O | hydrolysis product of endoxylanases on glucuronoxylan | Scheffersomyces stipitis | 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)[4-O-Me-GlcAalpha(1-2)]Xylbeta(1-4)Xyl | de-estrification of the methyl ester of MeGlcA with glucuronoyl esterase | ? | |
GlcAalpha(1-2)Xylbeta(1-4)Xylbeta(1-4)[GlcAalpha(1-2)]Xylbeta(1-4)Xyl + H2O | generated from the action of endoxylanases on polysaccharides | Scheffersomyces stipitis | alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)[GlcAalpha(1-2)]Xylbeta(1-4)Xyl | de-estrification of the methyl ester of MeGlcA with glucuronoyl esterase | ? |
Subunits | Comment | Organism |
---|---|---|
? | x * 120000, SDS-PAGE | Scheffersomyces stipitis |
Synonyms | Comment | Organism |
---|---|---|
alpha-glucuronidase | - |
Scheffersomyces stipitis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
protein unstable at this temperature with 50% activity loss in 30 min | Scheffersomyces stipitis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable for at least 3 h, at optimal temperature loss of 50% activity after 30 min | Scheffersomyces stipitis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.4 | - |
- |
Scheffersomyces stipitis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 5.5 | at pH 4.0 25.5% and at pH 5.5 51.6% activity | Scheffersomyces stipitis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Scheffersomyces stipitis | isoelectrofocusing on Multiphor II system using SEVALYT PRECOTES gels and markers from 3-10 | - |
4 |
Scheffersomyces stipitis | calculated from sequence | - |
4.6 |