Literature summary for 3.2.1.139 extracted from
Kiryu, T.; Nakano, H.; Kiso, T.; Murakami, H.
Immobilization of alpha -glucuronidase specific for O-alpha -D-glucosyluronic acid alpha -D-glucosiduronic acid from Aspergillus niger and production of D-glucuronic acid (2006), J. Appl. Glycosci., 53, 181-185.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
additional information |
glutaraldehyde stabilizes |
Aspergillus niger |
|
Application
Application |
Comment |
Organism |
additional information |
immobilized enzyme is effective in high yield production of D-glucuronic acid |
Aspergillus niger |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
D-glucuronic acid |
significantly inhibits in the presence of over 200 mM substrate, complete hydrolysis of 500 mM substrate is accomplished when D-glucuronic acid concentration is kept below 300-400 mM |
Aspergillus niger |
|
Organism
Organism |
UniProt |
Comment |
Textmining |
Aspergillus niger |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
partially purified by gel filtration |
Aspergillus niger |
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
61 |
- |
partially purified enzyme |
Aspergillus niger |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
O-alpha-D-glucosyluronic acid alpha-D-glucosiduronic acid |
the immobilized enzyme completely hydrolyzes 100 mM of the substrate |
Aspergillus niger |
D-glucuronic acid |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
TreDCase |
- |
Aspergillus niger |
Temperature Optimum [°C]
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
40 |
- |
immobilized TreDCase |
Aspergillus niger |
Temperature Stability [°C]
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
additional information |
- |
below 40°C, immobilized TreDCase, treatment with glutaraldehyde does not affect thermostability |
Aspergillus niger |
pH Optimum
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
additional information |
- |
3.0-3.5, immobilized TreDCase |
Aspergillus niger |
pH Stability
pH Stability |
pH Stability Maximum |
Comment |
Organism |
2 |
4 |
immobilized TreDCase |
Aspergillus niger |