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Literature summary for 3.2.1.139 extracted from

  • Suresh, C.; Rusd, A.A.; Kitaoka, M.; Hayashi, K.
    Evidence that the putative alpha-glucosidase of Thermotoga maritima MSB8 is a pNP alpha-D-glucuronopyranoside hydrolyzing alpha-glucuronidase (2002), FEBS Lett., 517, 159-162.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

Inhibitors

Inhibitors Comment Organism Structure
DTT 50 mM, required for maximal activity Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
p-nitrophenyl-alpha-D-glucuronopyranoside
-
Thermotoga maritima

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ slightly increases activity, 1 mM required for maximal activity Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
MSB8
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
MSB8
-

Purification (Commentary)

Purification (Comment) Organism
recombinant Thermotoga maritima

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-alpha-D-glucuronopyranoside + H2O
-
Thermotoga maritima p-nitrophenol + D-glucuronate
-
?
p-nitrophenyl-alpha-D-glucuronopyranoside + H2O
-
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 p-nitrophenol + D-glucuronate
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0018
-
p-nitrophenyl-alpha-D-glucuronopyranoside
-
Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
-
Thermotoga maritima

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 11 room temperature, 30 min, stable Thermotoga maritima
11.5
-
room temperature, 30 min, 50% loss of activity Thermotoga maritima