Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
DTT | 50 mM, required for maximal activity | Thermotoga maritima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
p-nitrophenyl-alpha-D-glucuronopyranoside | - |
Thermotoga maritima |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | slightly increases activity, 1 mM required for maximal activity | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
MSB8 | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | - |
MSB8 | - |
Purification (Comment) | Organism |
---|---|
recombinant | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
p-nitrophenyl-alpha-D-glucuronopyranoside + H2O | - |
Thermotoga maritima | p-nitrophenol + D-glucuronate | - |
? | |
p-nitrophenyl-alpha-D-glucuronopyranoside + H2O | - |
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 | p-nitrophenol + D-glucuronate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermotoga maritima |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
p-nitrophenyl-alpha-D-glucuronopyranoside | - |
Thermotoga maritima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Thermotoga maritima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | room temperature, 30 min, stable | Thermotoga maritima |
11.5 | - |
room temperature, 30 min, 50% loss of activity | Thermotoga maritima |