Literature summary for 3.2.1.139 extracted from

Biely, P.; Hirsch, J.; la Grange, D.C.; van Zyl, W.H.; Prior, B.A.
A chromogenic substrate for a beta-xylosidase-coupled assay of alpha-glucuronidase (2000), Anal. Biochem., 286, 289-294.

Search for more literature for 3.2.1.139

Application

Application	Comment	Organism
analysis	development of a precise alpha-glucuronidase assay by coupling the alpha-glucuronidase catalyzed formation of 4-nitrophenyl beta-D-xylopyranoside with its efficient hydrolysis by xylosidase. A recombinant strain of Saccharomyces cerevisiae, harboring and expressing the beta-xylosidase gene xlnD of Aspergillus niger under control of the alcohol dehydrogenase II promoter on a multicopy plasmid, is used as source of beta-xylosidase	Trichoderma reesei
analysis	development of a precise alpha-glucuronidase assay by coupling the alpha-glucuronidase catalyzed formation of 4-nitrophenyl beta-D-xylopyranoside with its efficient hydrolysis by xylosidase. A recombinant strain of Saccharomyces cerevisiae, harboring and expressing the beta-xylosidase gene xlnD of Aspergillus niger under control of the alcohol dehydrogenase II promoter on a multicopy plasmid, is used as source of beta-xylosidase	Aureobasidium pullulans
analysis	development of a precise alpha-glucuronidase assay by coupling the alpha-glucuronidase catalyzed formation of 4-nitrophenyl beta-D-xylopyranoside with its efficient hydrolysis by xylosidase. A recombinant strain of Saccharomyces cerevisiae, harboring and expressing the beta-xylosidase gene xlnD of Aspergillus niger under control of the alcohol dehydrogenase II promoter on a multicopy plasmid, is used as source of beta-xylosidase	Aspergillus tubingensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.21	-	4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside	37°C, pH 5.0	Aureobasidium pullulans
0.39	-	4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside	37°C, pH 5.0	Trichoderma reesei
0.58	-	4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside	37°C, pH 5.0	Aspergillus tubingensis

Organism

Organism	UniProt	Comment	Textmining
Aspergillus tubingensis	-	-	-
Aureobasidium pullulans	-	-	-
Trichoderma reesei	-	RUT C-30	-
Trichoderma reesei RUT C-30	-	RUT C-30	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside + H2O	-	Trichoderma reesei	4-nitrophenyl beta-D-xylopyranoside + 4-O-methyl-alpha-D-glucopyranose	-	?
4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside + H2O	-	Aureobasidium pullulans	4-nitrophenyl beta-D-xylopyranoside + 4-O-methyl-alpha-D-glucopyranose	-	?
4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside + H2O	-	Aspergillus tubingensis	4-nitrophenyl beta-D-xylopyranoside + 4-O-methyl-alpha-D-glucopyranose	-	?
4-nitrophenyl 2-(4-O-methyl-alpha-D-glucopyranosyl)-beta-D-xylopyranoside + H2O	-	Trichoderma reesei RUT C-30	4-nitrophenyl beta-D-xylopyranoside + 4-O-methyl-alpha-D-glucopyranose	-	?

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Release 2023.1 (January 2023)