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Literature summary for 3.2.1.139 extracted from
- The structural basis for catalysis and specificity of the Pseudomonas cellulosa alpha-glucuronidase, GlcA67A (2002), Structure, 10, 547-556.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging-drop vapor diffusion method | Cellvibrio japonicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D365A | activity is 0.0001% of wild-type activity | Cellvibrio japonicus |
| D365C | activity is 0.0001% of wild-type activity | Cellvibrio japonicus |
| E292A | activity is 0.0001% of wild-type activity | Cellvibrio japonicus |
| E292C | activity is 0.0001% of wild-type activity | Cellvibrio japonicus |
| E393A | activity is 0.0001% of wild-type activity | Cellvibrio japonicus |
| E393C | activity is22% of wild-type activity | Cellvibrio japonicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.28 | - |
aldotriouronic acid | - |
Cellvibrio japonicus |  |
| 0.36 | - |
aldotetraouronic acid | - |
Cellvibrio japonicus | |
| 0.42 | - |
aldopentaouronic acid | - |
Cellvibrio japonicus | |
| 0.53 | - |
aldobiouronic acid | - |
Cellvibrio japonicus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cellvibrio japonicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant | Cellvibrio japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| aldobiouronic acid + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| aldopentaouronic acid + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| aldotetraouronic acid + H2O | - |
Cellvibrio japonicus | ? | - |
? | |
| aldotriouronic acid + H2O | - |
Cellvibrio japonicus | 4-O-methyl-alpha-D-glucuronic acid + Xylbeta(1-4)Xylbeta(1-4)Xyl | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GlcA67A | - |
Cellvibrio japonicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 69.2 | - |
aldobiouronic acid | - |
Cellvibrio japonicus | |
| 96 | - |
aldotriouronic acid | - |
Cellvibrio japonicus | |
| 102.5 | - |
aldotetraouronic acid | - |
Cellvibrio japonicus | |
| 131.9 | - |
aldopentaouronic acid | - |
Cellvibrio japonicus | |
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