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Literature summary for 3.2.1.139 extracted from
- Purification and characterization of the alpha-glucuronidase from Thermoanaerobacterium sp. strain JW/SL-YS485, an important enzyme for the utilization of substituted xylans (1995), Appl. Environ. Microbiol., 61, 1077-1081.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.76 | - |
4-O-Methyl-glucuronosyl-xylobiose | - |
Thermoanaerobacterium saccharolyticum |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell associated | - |
Thermoanaerobacterium saccharolyticum | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74000 | - |
2 * 74000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
| 130000 | - |
gel filtration, native PAGE | Thermoanaerobacterium saccharolyticum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Thermoanaerobacterium saccharolyticum | important enzyme for the utilization of substituted xylans | ? | - |
? | |
| additional information | Thermoanaerobacterium saccharolyticum | enzyme is induced by growth on beech wood or birch wood | ? | - |
? | |
| additional information | Thermoanaerobacterium saccharolyticum JW/SL-YS485 | important enzyme for the utilization of substituted xylans | ? | - |
? | |
| additional information | Thermoanaerobacterium saccharolyticum JW/SL-YS485 | enzyme is induced by growth on beech wood or birch wood | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoanaerobacterium saccharolyticum | - |
- |
- |
| Thermoanaerobacterium saccharolyticum JW/SL-YS485 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoanaerobacterium saccharolyticum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
- |
Thermoanaerobacterium saccharolyticum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-methyl-D-glucuronosylxylan + H2O | - |
Thermoanaerobacterium saccharolyticum | 4-O-methyl-D-glucuronate + xylan | - |
? | |
| 4-O-methyl-D-glucuronosylxylan + H2O | - |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 | 4-O-methyl-D-glucuronate + xylan | - |
? | |
| 4-O-methyl-glucuronosyl-xylobiose + H2O | - |
Thermoanaerobacterium saccharolyticum | 4-O-methyl-glucuronate + xylobiose | - |
? | |
| 4-O-methyl-glucuronosyl-xylotetraose + H2O | - |
Thermoanaerobacterium saccharolyticum | 4-O-methyl-glucuronate + xylotetraose | - |
? | |
| 4-O-methyl-glucuronosyl-xylotetraose + H2O | - |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 | 4-O-methyl-glucuronate + xylotetraose | - |
? | |
| 4-O-methyl-glucuronosyl-xylotriose + H2O | - |
Thermoanaerobacterium saccharolyticum | 4-O-methyl-glucuronate + xylotriose | - |
? | |
| 4-O-methyl-glucuronosyl-xylotriose + H2O | - |
Thermoanaerobacterium saccharolyticum JW/SL-YS485 | 4-O-methyl-glucuronate + xylotriose | - |
? | |
| additional information | important enzyme for the utilization of substituted xylans | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
| additional information | enzyme is induced by growth on beech wood or birch wood | Thermoanaerobacterium saccharolyticum | ? | - |
? | |
| additional information | important enzyme for the utilization of substituted xylans | Thermoanaerobacterium saccharolyticum JW/SL-YS485 | ? | - |
? | |
| additional information | enzyme is induced by growth on beech wood or birch wood | Thermoanaerobacterium saccharolyticum JW/SL-YS485 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 74000, SDS-PAGE | Thermoanaerobacterium saccharolyticum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Thermoanaerobacterium saccharolyticum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | 65 | 45°C: about 80% of maximal activity, 65°C: about 85% of maximal activity | Thermoanaerobacterium saccharolyticum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
pH 6.2-7.9, stable for 1 h | Thermoanaerobacterium saccharolyticum |
| 62 | - |
half-life: 1 h | Thermoanaerobacterium saccharolyticum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Thermoanaerobacterium saccharolyticum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.4 | - |
- |
Thermoanaerobacterium saccharolyticum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 6.2 | pH 5: about 85% of maximal activity, pH 6.2: about 65% of maximal activity | Thermoanaerobacterium saccharolyticum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 7.9 | 60°C, stable for 1 h | Thermoanaerobacterium saccharolyticum |
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