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Literature summary for 3.2.1.139 extracted from
- alpha-(4-O-Methyl)-D-glucuronidase activity produced by rumen anaerobic fungus Piromonas communis a study of selected properties (1995), Appl. Microbiol. Biotechnol., 43, 893-900.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Piromyces communis | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 100000 | 300000 | MW ranging from 100000 to 300000, the fraction with the highest activity has a MW of 103000, gel filtration | Piromyces communis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Neocallimastix frontalis | - |
- |
- |
| no activity in Neocallimastix patriciarum | - |
- |
- |
| Piromyces communis | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | of the enzyme grown in cultures containing filter paper, barley straw, birchwood xylan or birchwood sawdust as carbon source | Piromyces communis | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-O-methyl-glucuronosyl-xylobiose + H2O | - |
Piromyces communis | 4-O-methyl-glucuronate + xylobiose | - |
? |  |
| 4-O-methyl-glucuronosyl-xylose + H2O | - |
Piromyces communis | 4-O-methyl-glucuronate + xylose | - |
? | |
| 4-O-methyl-glucuronosyl-xylotriose + H2O | - |
Piromyces communis | 4-O-methyl-glucuronate + xylotriose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Piromyces communis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
pH 5.5, in absence of substrate, 15 min, 20% loss of activity | Piromyces communis |
| 50 | - |
pH 5.5, absence of substrate, 15 min, 79% loss of activity | Piromyces communis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Piromyces communis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.8 | 6.3 | pH 5.8: about 80% of maximal activity, pH 6.3: about 50% of maximal activity | Piromyces communis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
40°C, absence of substrate, 20% loss of activity | Piromyces communis |
| 5.5 | - |
50°C, absence of substrate, 15 min, 79% loss of activity | Piromyces communis |
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