Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 97% inhibition at 1 mM | Cellulomonas sp. | |
Cu2+ | 88% inhibition at 1 mM | Cellulomonas sp. | |
EDTA | complete inhibition at 1 mM | Cellulomonas sp. | |
Hg2+ | 98% inhibition at 1 mM | Cellulomonas sp. | |
Zn2+ | 68% inhibition at 1 mM | Cellulomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
D-mannan | pH 7, 37°C, hydrolysis of alpha-1,2-linkage in yeast mannan | Cellulomonas sp. | |
3.2 | - |
D-mannan | pH 7, 37°C, hydrolysis of alpha-1,6-linkage in yeast mannan | Cellulomonas sp. | |
200 | - |
D-mannan | pH 7, 37°C, hydrolysis of alpha-1,3-linkage in yeast mannan | Cellulomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
450000 | - |
gel filtration | Cellulomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannan + H2O | Cellulomonas sp. | hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose | D-mannose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas sp. | - |
production of the enzyme strongly induced by yeast extract, 2 different MW forms | - |
Purification (Comment) | Organism |
---|---|
high molecular weight form | Cellulomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.8 | - |
- |
Cellulomonas sp. |
Storage Stability | Organism |
---|---|
-20°C | Cellulomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-mannan | hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose, hydrolysis rate of alpha-1,3-linkage is very low | Cellulomonas sp. | D-mannose | - |
? | |
D-mannan + H2O | hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose | Cellulomonas sp. | D-mannose | - |
? | |
additional information | only slight activity towards 4-nitrophenyl-alpha-D-mannoside and no hydrolysis of O-alpha-D-mannopyranosyl-1,2-D-mannitol | Cellulomonas sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cellulomonas sp. |
50 | - |
- |
Cellulomonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
10 min, stable | Cellulomonas sp. |
50 | - |
10 min, 50% loss of activity | Cellulomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | - |
Cellulomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 9 | pH 5: about 20% of activity maximum, pH 9: about 15% of activity maximum | Cellulomonas sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 9.5 | 4°C, 48 h, stable | Cellulomonas sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Cellulomonas sp. | pH range 3.5-10 in a 0-60% sucrose gradient | - |
3.2 |