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Literature summary for 3.2.1.135 extracted from

  • Jung, J.H.; An, Y.K.; Son, S.Y.; Jeong, S.Y.; Seo, D.H.; Kim, M.K.; Park, C.S.
    Characterization of a novel extracellular alpha-amylase from Ruminococcus bromii ATCC 27255 with neopullulanase-like activity (2019), Int. J. Biol. Macromol., 130, 605-614 .
    View publication on PubMed
Search for more literature for 3.2.1.135

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Ruminococcus bromii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 pullulan Km-value: 15.4 mg/ml, 45°C, pH 5.0 Ruminococcus bromii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Ruminococcus bromii
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pullulan + H2O Ruminococcus bromii the enzyme may have a role to provide branched maltooligosaccharides to stimulate the growth of beneficial microorganisms in the human intestine panose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Ruminococcus bromii
-
-
-
Ruminococcus bromii ATCC 27255
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Ruminococcus bromii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
72.5
-
 45°C, pH 5.0 Ruminococcus bromii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pullulan + H2O the enzyme may have a role to provide branched maltooligosaccharides to stimulate the growth of beneficial microorganisms in the human intestine Ruminococcus bromii panose + ?
-
 ?
pullulan + H2O the enzyme is an alpha-amylase with neopullulanase-like activity Ruminococcus bromii panose + ?
-
 ?

Synonyms

Synonyms Comment Organism
Rbamy5
-
 Ruminococcus bromii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
297
-
 pullulan 45°C, pH 5.0 Ruminococcus bromii

General Information

General Information Comment Organism
physiological function the enzyme may have a role to provide branched maltooligosaccharides to stimulate the growth of beneficial microorganisms in the human intestine Ruminococcus bromii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
19.3
-
 pullulan 45°C, pH 5.0 Ruminococcus bromii