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Literature summary for 3.2.1.133 extracted from

  • Ben Mabrouk, S.; Ayadi, D.Z.; Ben Hlima, H.; Bejar, S.
    Thermostability improvement of maltogenic amylase MAUS149 by error prone PCR (2013), J. Biotechnol., 168, 601-606.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli DH5alpha cells Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants Comment Organism
D46V/P78L/V145A/K548E the half-life times for the mutant at 50°C and 55°C are 70 min and 25 min, compared to 30 min and 13 min for the wild type, respectively Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
starch + H2O Bacillus sp. (in: Bacteria)
-
?
-
?
starch + H2O Bacillus sp. (in: Bacteria) US149
-
?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) A7DWA8
-
-
Bacillus sp. (in: Bacteria) US149 A7DWA8
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and UNO-Q12 anion exchange column chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-cyclodextrin + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
beta-cyclodextrin + H2O
-
Bacillus sp. (in: Bacteria) US149 ?
-
?
pullulan + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
pullulan + H2O
-
Bacillus sp. (in: Bacteria) US149 ?
-
?
starch + H2O
-
Bacillus sp. (in: Bacteria) ?
-
?
starch + H2O
-
Bacillus sp. (in: Bacteria) US149 ?
-
?

Synonyms

Synonyms Comment Organism
maltogenic amylase
-
Bacillus sp. (in: Bacteria)
MAUS149
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Bacillus sp. (in: Bacteria)

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55 more than 50% activity between 20 and 50°C, at 55°C the enzyme retains 38% of activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 55 the half-life times for the wild type enzyme at 50°C and 55°C are 30 min and 13 min, respectively Bacillus sp. (in: Bacteria)