Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Bacillus sp. (in: Bacteria) |
Protein Variants | Comment | Organism |
---|---|---|
D46V/P78L/V145A/K548E | the half-life times for the mutant at 50°C and 55°C are 70 min and 25 min, compared to 30 min and 13 min for the wild type, respectively | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
starch + H2O | Bacillus sp. (in: Bacteria) | - |
? | - |
? | |
starch + H2O | Bacillus sp. (in: Bacteria) US149 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | A7DWA8 | - |
- |
Bacillus sp. (in: Bacteria) US149 | A7DWA8 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and UNO-Q12 anion exchange column chromatography | Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
beta-cyclodextrin + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
beta-cyclodextrin + H2O | - |
Bacillus sp. (in: Bacteria) US149 | ? | - |
? | |
pullulan + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
pullulan + H2O | - |
Bacillus sp. (in: Bacteria) US149 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) US149 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
maltogenic amylase | - |
Bacillus sp. (in: Bacteria) |
MAUS149 | - |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | more than 50% activity between 20 and 50°C, at 55°C the enzyme retains 38% of activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | the half-life times for the wild type enzyme at 50°C and 55°C are 30 min and 13 min, respectively | Bacillus sp. (in: Bacteria) |